Zhao et al., "Arabidopsis ubiquitin conjugase
UBC32 is an ERAD component that functions in brassinosteroid-mediated salt stress tolerance," Plant Cell, vol.
Gene Gene name US patent symbol MGST3 Microsomal glutathione S-transferase 3 5,919,627 GBP1 Guanylate binding protein 1, interferon- 6,894,157 inducible, 67kDa GGH Gamma-glutamyl hydrolase (conjugase
, 5,801,031 folylpolygammaglutamyl hydrolase) MTAP Methylthioadenosine phosphorylase 5,942,393; 6,870,037 MADCAM1 Mucosal vascular addressin cell adhesion 7,750,137 molecule 1 POSTN Periostin, osteoblast specific factor 6,518,063 PMS1 PMS1 postmeiotic segregation increased 1 5,922,855; (S.
(1989), segun la cual en este periodo embrionario de un nuevo orden politico y economico en la region, y en el inicio del periodo siguiente, habrian ocurrido conflictos en diversos niveles hasta llegar a un equilibrio que conjugase
los intereses comunes y particulares de todos los grupos involucrados.
To release bound folate, alpha-amylase, protease and conjugase
were added to the extracts.
Samples were removed every 3 hr, heat-treated and treated with conjugase
before the microbiological assay was performed.
It is assumed that this procedure achieves two goals: the complete lysis of erythrocytes and the exposure of intracellular folate polyglutamates to plasma conjugase
enzyme ([gamma]-glutamyl hydrolase) at a pH near 4.5, which favors their conversion to assayable folate.
All lysates (including those that may have undergone preliminary freezing-thawing) were incubated for 2 h at 37 [degrees]C so that any released red cell folyl-polyglutamates could undergo deconjugation, with naturally occurring plasma folate conjugase
(y-Glu-X carboxypeptidase; EC 188.8.131.52), to the monoglutamate form, which is the appropriate form for assay.