We teased conglutinates apart with dissecting probes to free glochidia.
We used 25 rainbow darters and 25 fantail darters from the Cumberland River drainage, and 10 conglutinates from each of nine mussels for each infestation.
Forty conglutinates were removed from the Wolf River mussels (20 per mussel) and used to infest fish on the same day we infested fish with glochidia from the Clinch River for Trial 1 (18 Jan.
We used 10 conglutinates from each of nine mussels from the Clinch River to infest these species.
Developing embryos and outer conglutinates began to form by 7 Sept.
The number of conglutinates varied among individuals.
Ortmann (1910, 1911, 1912) reported the discharge of conglutinates in Ptychobranchus through the edge of the marsupium.
Luo (1993) noted that conglutinates of Ptychobranchus subtentum (Say, 1825) from the Tennessee River drainage resembled larval aquatic insects, and that the method of release was unknown.
The high level of mimicry exhibited by Ptychobranchus greeni conglutinates, and an unusual mechanism for maintaining conglutinates within host fish habitat, are selective traits to facilitate parasitism and survival of offspring.