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tuberculosis in vivo of small molecule in vivo^ is impaired by complete or partial loss of target function^ Random, allelic exchange, Animal model of infection or conditional mutant in and drug efficacy^^ target gene^^ Note: The criteria for each step are indicated with ^ and the experimental approaches applied in testing the criteria are indicated with ^^.
The development of systems for the conditional regulation of gene expression in MTB under in vitro and in vivo conditions offers an elegant solution to this problem, provides unprecedented insights into otherwise intractable metabolic pathways, and enables the simultaneous assessment of multiple conditional mutants in a single experiment (17)--a modification that holds promise for the development of high-throughput in vivo screens.
The research yielded the first collection of conditional mutant alleles outside the mouse; unlike popular mouse conditional alleles that are switched from "on" to "off," zebrafish mutants conditionally go from "off" to "on," offering new insight into localized gene requirements.
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