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In designing such experiments, it is most straightforward to use microorganisms, given their short generation time and readily available conditional lethal mutations.
Finally, it should be noted that the use of conditional lethals, while allowing observation of a microevolutionary change within a manageable period, may also unintentionally create or reinforce a belief that selection primarily occurs in gross mutants and in short time frames.
The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes.
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