Thirty microliter aliquots of the equilibrated cells were then preincubated in polypropylene tubes at 37[degrees]C in the presence of the test compounds (hydroxytyrosol or oleuropein), before incubation with concanavalin
A (final concentration 200 [micro]g/ml, with 50 [micro]g/ml phosphatidylserine added as a co-stimulator), compound 48/80 (final concentration 10 [micro]g/ml), or calcium ionophore A23187 (final concentration 50 [micro]g/ml), for 10 min at 37[degrees]C.
Orally administration of 25 to 100 mg sodium nitrite per kg diet to Balb/c mice for 21 d has resulted in a dose dependent decrease of lymphocyte percentages, concanavalin
A and lipopolysaccharide-induced lymphocyte proliferation, natural killer cell activity against WEHI-164 target cells, as well as IgM and IgG titers against injected sheep erythrocytes (Chow and Hong, 2002).
Astilbin also prevented concanavalin
A-induced liver injury by reducing tumor necrosis factor-[alpha] (TNF-[alpha]) production and T lymphocyte adhesion (9), and it significantly suppressed collagen-induced arthritis by causing the dysfunction of lymphocytes and inhibiting lymphocyte migration (10, 11).
Lipopolysacchride and concanavalin
A (ConA) were from Sigma (St.
Murine splenocytes placed in serum-free medium will be stimulated with concanavalin
A with or without phorbol 12-myristate 13-acetate (PMA).
Considering the application of the compounds in chemopreventive medicine, we examined their effects on normal human peripheral blood lymphocytes (PBLs) stimulated with concanavalin
A (Con-A) all compounds exhibited no marked growth inhibition at lower doses than 150p,M.
Lymphocyte mitogen concanavalin
A (Type IV, C-2010, Sigma St Louis, MO, USA) was added at a final concentration of 16 mg/ml of culture medium, after which the plates were incubated at 37[degrees]C in an incubator in an atmosphere of 5% C[O.
We previously developed a multilectin affinity system for efficient and specific enrichment of human serum glycoproteins (7) and demonstrated that a multilectin affinity chromatography (M-LAC)  column containing Jacalin, concanavalin
A, and wheat germ agglutinin could overcome the low affinity and lack of complete glycoprotein capture that typically occurs with a single-lectin affinity selector.
In some cases plant lectins have been shown to be able to act as opsonins in invertebrate phagocyte systems, and concanavalin
A receptors have been described on oyster hemocytes (Sami et al.
The cytochrome P450 aromatase was purified from equine microsomes, after its separation from reductase, by successive chromatographic steps: concanavalin
A-Sepharose 4B affinity column, diethyl amino ethyl-Sepharose CL-6B ion exchange, and hydroxyapatite-Sepharose 4B adsorption/partition columns (Moslemi et al.
The mitogen, lipopolysaccharide (LPS, Sigma, USA) or concanavalin
A (ConA, Sigma, USA), was added at a final concentration of 10 [micro]g/ml.
A mitogen called Concanavalin
A (ConA, Sigma, Israel) was used to stimulate the proliferation of the T-cells.