CAFSCs of the experimental group are cultured with neuron inductive medium containing 10%FBS, 1uM all-trans retinoic acid and 100 uM [beta]-mercaptoethanol while CAFSCs of control group are remained in complete medium
. Medium is refreshed every three days.
Cells were cultured in 100mm plates with 10ml complete medium
under the condition of 37AdegC and 5% CO2 in a humidified incubator.
hAECs were cultured in a high-glucose DMEM supplemented with 10% FBS (complete medium
), and hAECs at passage 2-3 were used.
An equal volume of a complete medium
comprising Dulbecco's modified Eagle medium (DMEM/F12; Hyclone, USA) and 10% fetal bovine serum (Gibco, Australia) was added, and the mixture centrifuged again at 800 xg for 10 min.
He said Punjab's vision was to revive growth and private sector investment to complete medium
term development framework 2014-18 and Punjab Growth Strategy to achieve the growth rate of 8 percent by 2018.
2012) were seeded into 4 wells of a 96-well flat-bottom plate at 5 x [10.sup.6] cell/ml in 100 [micro]l complete medium
, thereafter Con A (3 [micro]g/ml) or medium with MGEB were added giving a final volume of 200 [micro]l.
While serum-free media are well established, this is the first complete medium
available for the culture and expansion of hematopoietic stem cells that ?s free of animal and human proteins and only contains recombinant and synthetic components.
The new Khalidiya Center is equipped with two maintenance lines in order to complete medium
and small maintenance services with high quality and speed," he added.
Meat is a complete medium
for micro-organisms, hence a precious diet for human consumption.
In our study, we preferred effectene transfection reagent, since it shows low toxicity and provides transfection of small amounts of DNA, while the transfection procedure could be performed in the complete medium
Control and CAsE-PE (chronic-arsenic-exposed human prostate epithelial) cells were continuously maintained in a complete medium
[keratinocyte serum-free medium (K-SFM) with bovine pituitary extract and epidermal growth factor] or in a steroid-depleted medium (K-SFM alone).