complementation test


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complementation test

a genetical test to determine the precise location of mutations in one area of DNA. See CIS-TRANS TEST.
References in periodicals archive ?
To confirm the functional regions of scMed6p, larger deletion mutants were made and used in a complementation test. First, we constructed a Med6p mutant having the combined deletion of [DELTA]3 and [DELTA]4 ([DELTA]3-4), resulting in the removal of the first region specific for scMed6p.
Construction of the (A3)FL3(RpRf3rf4rf4) and (A3)FL4 (rf3rf3Rf4Rf4) lines and analogous (N)FL3 (Rf3Rf3rf4rf4) and (N)FL4 (rf3rf3Rf4Rf4) lines permitted a reciprocal complementation test. The restoration of pollen staining capability and of seed set in crosses made in either direction, (A3)FL4/(N)FL3 or (A3)FL3/ (N)FL4, is consistent with pollen staining and seed set of heterozygous Rf3rf3Rf4rf4 plants, and with the two-gene gametophytic model (Pring et al., 1999; Tang et al., 1998).