miRNAs have become the focus of many researchers because of their significant role in the degradation of mRNA, post-translational inhibition through complementary base pairing
(4), and ability to control many biological processes such as homeostasis (3).
The chemical base structure determines the principle of complementary base pairing
, which is known as the Watson-Crickbase pairing principle, namely, A bonds with T using two hydrogen atoms and G bonds with C using three hydrogen atoms [14,15].
Fusing two single stranded DNA molecules by complementary base pairing
is also called annealing.
The students already knew about complementary base pairing
from a previous chapter, so I had them collaborate in pairs to work on the base pairing for DNA, mRNA, and tRNA, noting the replacement of thymine (T) by uracil (U) in RNA.