Nakano, "Synthesis of oligonucleotide derivatives containing pyrene labeled glycerol linkers: enhanced excimer fluorescence on binding to a complementary DNA
sequence," Tetrahedron Letters, vol.
In 1978, he went to Stanford University School of Medicine as postdoctoral fellow and developed a new technique that enables the cloning of full-length complementary DNAs
on the basis of their functional expression in a given cell of interest.
cDNA (complementary DNA
): A segment of DNA identical in base sequence to at least part of the coding sequence of a gene, generated in the laboratory from a natural mRNA molecule, cDNA can be produced in large quantities for sequencing and other genetic studies.
DNA chips for examining gene expression involve longer fragments of synthetic or complementary DNA
. Applications of these chips include protein correlation studies, sequencing by hybridization and functional genomic applications.
In a method called expressed sequence tagging (EST), researchers isolate these RNA templates from cells and convert them back into a DNA form (complementary DNA
, or cDNA), which is then pasted into carriers.
Like many jumping genes, the P-element has two short, mirror-image sequences at either end, which are thought to facilitate a splicing reaction with the host's DNA; the host cell papers over the gaps with complementary DNA
She plans to take one of these RNA strands from the tomato spotted wilt virus, make a complementary DNA
, then reverse the direction of a portion of that DNA sequence.
The DNA-modified nanoparticles -- gold in this case -- are positioned on a pre-patterned template made of complementary DNA
. Stacks of structures can be made by introducing a second and then a third DNA-modified particle with DNA that is complementary to the subsequent layers.
The sensitivity of the proposed colorimetric assay method based on unmodified AuNPs for quantifying concentration of dengue virus DNA target was conducted through the hybridization of 12 [micro]M of PNA probe solutions with different complementary DNA
target concentration at ratios of 0, 0.01, 0.03, 0.05, 0.1, 0.5, and 1, respectively (Table 3).
In recent research, scientists used a genetic therapy in laboratory mice, in which a virus helped carry complementary DNA
into appropriate cells and restored some GluN2B subunits.
Supreme Court on June 13 ruled that companies cannot patent isolated human genes, but can patent human-engineered DNA molecules, including so-called complementary DNA
, or cDNA, can be patented as it does not occur naturally.