Bruns, S., Stark, Y., Roker, S., Wieland, M., Drager, G., Kirschning, A., Stahl, F., Kasper, C., Scheper, T., 2007, "Collagen biomaterial doped with colominic acid
for cell culture applications with regard to peripheral nerve repair," J.
Briefly, each virus variant was mixed with 5-200 [micro]g/mL of colominic acid ([alpha]2,8-linked polysialic acid) and incubated for 1 h at 4[degrees]C.
We performed other inhibition assays and observed that [HN-K.sub.335] possesses a higher affinity towards [alpha]2,8-linked sialic acids (colominic acid), which was evidenced by competence with colominic acid (Figure 1).
Hemolysis depends on the sialic acid-binding activity of limulin, because sialylated glycoconjugates, such as fetuin and the sialic acids N-acetyl neuraminic acid and colominic acid
, inhibit hemolysis, and desialylation of the target cells renders them immune to cytolysis (1).