They are used as energy source, cell membrane recovery, hormones among other functions (Hide et al., 1992); however, the hydrolysis of lipids is highly complex by lipases due the interface water-lipid that implies the necessity for the enzyme to join to colipase
and bile salt to break the ester bonds (Gjellesvik et al., 1992).
Also included herein is the digestive enzyme colipase
 and the protein Dickkopf [3, 17, 18], which is an inhibitor of Wnt signaling.
The 2.46 AA(R) resolution structure of the pancreatic lipase colipase
complex inhibited by a C11 alkyl phosphonate.
Putative association between a new polymorphism in exon 3 (Arg109Cys) of the pancreatic colipase
gene and type 2 diabetes mellitus in two independent Caucasian study populations.
The importance of bile salt for the reactivation of pancreatic lipase by colipase
. Biochim Biophys Acta 750: 171-7.
Olive oil was utilised as substrate in sodium taurodeoxicolate 6mM, NaCl 0.15M, Ca[Cl.sub.2] 1mM, Tris (hydroxymethyl) aminomethane buffer 0.2mol [L.sup.-1] pH 8.0 and partially purified broiler colipase
Secretion of pancreatic lipase and colipase
from rat pancreas.
(2) However, contemporary lipase assays now contain colipase
and bile salts that increase the analytical sensitivity of lipase assays.
All four experimental methods (experimental reagents from Sentinel) contained colipase
, but differed from one another in stabilizing proteins and in the size of substrate micelles: two methods contained protein (0.3 g/L) and micelles with either a 250-nn (P250) or 400-nn (P400 method) diameter, respectively; the two methods without added protein used micelles with either a 250-nn (NP250) or 400-nn (NP400) diameter.
Serum lipase activity increased in all our patients without a parallel increase in serum amylase titers; the combination of accurate clinical, biochemical, and instrumental study of these patients allowed us to exclude the possibility of a normoamylasemic pancreatic injury, and the presence of appropriate concentrations of bile salts and colipase
in the reagent favors a full and specific action of lipase assay, at the same time inhibiting serum esterase (8).
In the small intestine lipolysis is completed by pancreatic lipase acting with colipase
as cofactor and absorption of lipolytic products starts.