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performed a randomized trial of the vasodilator fenoldopam in 17 deceased-donor kidney transplant patients with cold ischemia time >12 hours [42].
Studies have shown that cold ischemia time does not influence graft survival in pediatric renal transplantations22,26,27, a matter which is in line with our findings.
It was our institution's intention to use pulsatile perfusion in all DCD kidneys with a predicted cold ischemia time of 6 hours or greater.
Eliminating pretransplant crossmatching in certain transplant recipients can shorten cold ischemia time and save money, according to a report in the August issue of Clinical Transplantation.
DNA extracted from FFPE tissue that was subjected to a cold ischemia time of 1 hour displayed reduced fluorescent in situ hybridization (FISH) signals, (5) although a cold ischemia time of 24 hours did not alter PCR amplification success rates (data not shown).
Concerning traditional predictors of DGF and except for cold ischemia time, no significant differences were found between DGF/non-DGF in relation to baseline characteristics and induction therapy (Table 1).
The recorded variables included the number of HLA-A, HLA-B and HLA-DR mismatches, maximum and most recent titres of panel reactive antibodies, cold ischemia time, age and gender of donor and recipient, and recipient weight and race.
Delayed graft function appeared to be affected more by cold ischemia time than by the number of HLA mismatches.
Not surprisingly, we have also learned that cold ischemia time is important to the viability of some antigens.
Because of increasing recognition of the importance of cold ischemia time, particularly for organs of marginal quality, the UNOS Liver and Intestinal Organ Transplantation Committee and Pediatric Transplantation Committee anticipate that the size of the distribution unit would be limited to minimize cold ischemia time while providing a median waiting time less than the expected median survival time for any level of disease severity.
(Refer to the OPTN reference for further discussion of KDPI.) Other factors considered include ages of both donor and recipient and their comorbidities, donor characteristics that included warm and cold ischemia times (CIT)--the length of time the organ is perfused with a cold preservation solution until implantation and re-establishment of vascular flow, and the donor service area.