colcemid


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Colcemid

 [kol´sĕ-mid]
trademark for a preparation of demecolcine, a cytotoxic alkaloid used in chromosome analysis.

colcemid

a compound related in structure and function to colchicine.
References in periodicals archive ?
Reagents for molecular biology: taq polymerase, colcemid and pcr purification kit for sequencing products.
BM samples were cultured for 24-48 hours in RPMI-1640 medium (supplemented with %10 fetal calf serum and antibiotics) without mitogen stimulation and with 10[micro]g/ml colcemid concentration.
High-resolution cultures were initiated in RPMI media with GlutaMAX (Gibco) and treated with 5-fluoro-2'-deoxyuridine 48 hours after culture initiation and ethidium bromide and colcemid at the time of harvest.
1 [micro]g/mL colcemid (GIBCO-BRL, Grand Island, NY, USA) to the complete medium for the last 3 hr before harvest.
After 2-days, the cells were incubated with 15 [micro]l of colcemid (KARYO MAX[R] COLCEMID[R] Solution) for 24 h in a humidified environment of 5% C[O.
1 ml colcemid solution (10 [micro]g/ml) was added to each culture tube and mixed by shaking gently.
1mL of colcemid, the cultures were removed later and were decanted into l5mL centrifuges tubes.
Methyl colchicine, colcemid, which differs from colchicine by having an N-methyl group instead of a N-acetyl group, is widely used by animal cell workers but less so by plant cytologists, and the same is true of the alkaloid vinblastine.
Pgp acts to rapidly pump a large number of hydrophobic and heterocyclic cancer chemotherapeutic agents including adriamycin (doxorubicin), colchicine, colcemid, etoposide, taxol, vincristine and vinblastine out of the tumor cells, thereby preventing accumulation within the cells of effective levels of the chemotherapeutic agents.
3 [micro]g of colcemid (N-deacetyl-Nmethylcolchicine; Gibco BRL, USA) was added to arrest cell division.