A functional repressor binding site has not previously been inserted into the MLP in situ for the regulation of its expression in an adenovirus genome, primarily because the virus DNA polymerase coding sequence
is in the opposing DNA strand.
AG013 is an oral mouth rinse composed of a recombinant Lactococcus lactis bacteria strain that contains the coding sequence
for human trefoil family factor 1 (hTFF1), which is continually secreted by the bacteria.
Using plasmids pBHA and pCAG-EGFPd2 (a gift from Connie Cepko) (21) and Addgene plasmid # 14760 as the templates, human [beta]-globin (HBG) 5'-3'UTRs, and the coding sequence
of destabilized green fluorescent protein (EGFPd2) were amplified by specific primers.
The genome contains genes, which are regions of DNA which get transcribed to RNA; in some cases this RNA is itself directly functional (things like tRNAs or the 18S component of the ribosome, for instance) but in most cases, the RNA is an mRNA, carrying a protein coding sequence
which is translated by the ribosomal machinery into a covalently attached series of amino acids--a protein--which by nature of the varied side chain chemistries and their electrostatic, hydrogen bond, and hydrophobic interactions folds up to a thermodynamic energy minima state to create a functional enzyme or structural protein.
VM202 is a novel genomic cDNA hybrid human hepatocyte growth factor gene with a novel and proprietary coding sequence
(HGF-X7) expressing two isoforms needed for optimal therapeutic benefits.
DNA's coding sequence
is described by four base pairs: cytosine, guanine, adenine, and thymine.
Typically, these receptors are used to graft the specificity of a monoclonal antibody onto a T cell, with transfer of their coding sequence
facilitated by retroviral vectors.
For the construction of the plasmid containing the whole PpPbp gene fused to the GFP gene (PpPbp-GFP), the coding sequence
of PpPbp was amplified by PCR from the pBSPpPbp plasmid with the PpPbp(GFP)-F-SalI and PpPbp(GFP)-R2-SalI primers.
The coding sequence
is 1539 bp long which is similar to its ortholog in tomato.
The coding sequence
region of CK-M gene was sequenced for comparison analysis with other species.
They then moved on to simulating the operation of a vending machine which taught them about coding sequence
, selection and looping.
The RNA used in this experiment contained the coding sequence
for TERT, the active component of a naturally occurring enzyme called telomerase.