Use of polyvalent coagglutination
reagents for serotyping of Streptococcus suis.
We investigated capsular expression of the retrieved NL119 by coagglutination
tests and used the colony giving the strongest reaction within 30 s for the subsequent in vivo passage.
Nonculture methods now available, such as counterimmunoelectrophoresis (CIE) and coagglutination
, have failed to show uniform diagnostic sensitivity or specificity even when applied to specimens collected from patients with culture-confirmed bacteremic pneumococcal pneumonia [5,6] (1) The lack of sensitive nonculture-based methods and the limitations of culture often result in only presumptive diagnoses of pneumococcal pneumonia .
Other developments include the use of the limulus assay lysate test for endotoxins; measurement of bacterial ATP by bioluminescence; rapid detection of bacterial antigens, toxins, and antibodies by coagglutination
techniques and enzyme immunoassay; and rapid identification of bacteria and its products by high-pressure liquid chromatography and head-space chromatography.
suis isolates using PCR (10) and confirmed serotypes by coagglutination
using rabbit antiserum (Statens Serum Institut, Copenhagen, Denmark).
Therefore, the species and serotype were identified by sequence analysis of a 16S rRNA gene and a coagglutination
test as described (4,5).
suis isolate from this patient was identified as serotype 2 by coagglutination
test at the International Reference Laboratory at the Universite de Montreal (5).
The final serotype of all strains was confirmed by coagglutination
tests that used rabbit antiserum (Statens Serum Institute, Copenhagen, Denmark).
The isolate was assigned to serotype 2 by the coagglutination
test (7) and shown by Western blot to produce suilysin, extracellular protein factor and muramidase-released protein, which are virulence markers often associated with highly virulent strains of Eurasian, but not North American, origin (1,8).
CAMP Test, Serotyping, Antimicrobial Susceptibility, and Pulsed-Field Gel Electrophoresis Identification of the isolates was confirmed by CAMP testing, and serotype determination was achieved by using a coagglutination
typing kit (Essum, Bacterium AB, Umea, Sweden).
suis serotyping, where it was identified by the coagglutination
test as serotype 14 (5).
Serotyping and subtyping of Neisseria meningitidis isolates by coagglutination
, dot blotting and ELISA.