Furthermore, histopathological analysis and hematopoietic progenitor clonogenic assay
of tissues collected show a significant increase in bone marrow cell numbers and improved regenerative capability into all blood lineages.
We further determined the roles of Grail in tumorigenesis by clonogenic assay
in KB/Grail and KB/shGrail cells.
Cell lines with amplified DSCC1 locus are dramatically affected in clonogenic assay
by DSCC1 silencing, while cell lines without DSCC1 amplification maintain normal clonogenic ability after DSCC1 silencing.
For the clonogenic assay
, 1 x [10.sup.3] cells were plated in 60-mm dishes and cultured in medium containing DMSO or oridonin for 2 to 3 weeks.
To confirm the capacity of selected proinflammatory cytokines to drive the HSPCs toward a myeloid lineage, we performed clonogenic assay
The values of SER10 were calculated from the curves of clonogenic assay
. The enhanced in vitro and in vivo radiosensitization effect and possible underlying mechanism were reported for the first time, which solidified the promising application of curcumin loaded PVP-PCL nanoparticles as a novel radiosensitizer.
Results of clonogenic assay
(CFU) and flow cytometry analyses (FACS) of mesenchymal stem cells (MSCs, (b)), endothelial progenitor cells (EPCs, (c), (d)), and hematopoietic stem cells (HSCs, (e), (f)).
To determine the long-term cytotoxicity effects of apigenin, a clonogenic assay
was used .
. A clonogenic assay
was performed to determine the effects of STS treatment on the survival and division of the radiation-treated H9c2 cells.
. Cells were plated in 60 mm dishes (1000/dish) and were treated with different concentrations of wogonin or MPP for 48 hours.
The clonogenic assay
determines the ability of a cell to proliferate indefinitely, thereby retaining its reproductive ability to form a large colony or a clone.
. For the clonogenic assay
, HEK293T cells were seeded into six-well dishes at 500 cells/well and incubated overnight in complete medium.