Current research showed enhanced
clonogenic potential of diabetic-ASCs exposed to bFGF in 5 ng/mL concentration; nonetheless this effect was lost, when the dose of the stimuli was increased to 10 ng/mL.
Cytotoxicity, Antioxidant, and
Clonogenic Survival Activities and Inhibitory Activities on Angiogenesis and Cell Invasion.
Clonogenic survival assay revealed that the ability of clone formation was decreased in the si-SIRT1 cells by 36.5%, comparing with the NC group (Figures 3(a) and 3(b)).
This combination showed potent growth inhibition with ~2.5 fold of dose advantage and also significantly decreased the wound healing and
clonogenic potential of breast cancer cells.
[6] The stromal cells may produce inhibitory factors which suppress
clonogenic potential of mesenchymal stem cells.
The
clonogenic assay in HEK293T cells identified positive proliferation and cellular survival after treatment with 0.5 [micro]M [As.sub.2][O.sub.3] for 15 days (Figure 1L,M).
Another study evaluated Rhus verniciflua Stokes inhibition of the
clonogenic growth of small numbers of UACC-812 breast cancer cells cocultured with fibroblasts in vitro [16].
Clonogenic cell survival assay was used in our study to determine antiproliferative effects of AA extracts by checking its ability to inhibit cancer cells from dividing and forming colonies.
Only a small subset of cells is known to have stem-cell-like property and 1% of viable cells were assumed to be
clonogenic cells in the model [23].
They described these cells as multipotential stromal precursor cells, which were spindle shaped and
clonogenic in culture conditions, defining them as colony-forming unit fibroblasts (CFU-F).
Seandel et al, have identified GPR125 as a surface marker for self renewing
clonogenic spermatogonial progenitor cells in mouse (6).
In the present study, we isolated and purified rabbit BMSCs based on their ability to form adherent
clonogenic cell clusters.