cloning vector


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Related to cloning vector: expression vector

clon·ing vec·tor

an autonomously replicating plasmid or phage with regions that are not essential for its propagation in bacteria and into which foreign DNA can be inserted; this foreign DNA is replicated and propagated as if it were a normal component of the vector.
A recombinant DNA molecule containing sequences from 2 or more sources—e.g., plasmids, bacteriophage, cosmids—into which foreign DNA—40+ kb—may be inserted for cloning; a DNA sequence capable of self-replication in a suitable host cell, which has site(s) for inserting DNA fragments by recombinant DNA techniques, and genetic markers that allow selection for the vector in a host cell; CVs usually have a selective marker—e.g., for antibiotic resistance—and a site for cleavage by a restriction endonuclease

clon·ing vec·tor

(klōn'ing vek'tŏr)
An autonomously replicating plasmid or phage with regions that are not essential for its propagation in bacteria and into which foreign DNA can be inserted; this foreign DNA is replicated and propagated as if it were a normal component of the vector.

cloning vector

see VECTOR.
References in periodicals archive ?
In order to confirm that cellulase gene was successfully inserted in the pTZ57R/T cloning vector, colony PCR was performed.
Purified PCR product was cloned into STV28 cloning vector. Nucleotide sequence of the cloned gene was approved and deposited in gene bank after sequencing.
The amplified fragments of exochitinase A gene were ligated in pTZ57R/T cloning vector and were used for transformation of E.
The nFGH gene fragment was cloned into pGEM-T cloning vector (Promega, USA) and transformed into E.
These have been cloned into a standard BAC cloning vector. An estimate of the average cloned insert size = 125kb.
After size-fractioning of products in agarose gels, each product was purified (Wizard[R] SV gel and PCR clean-up system --Promega) and cloned into pGEM-T Easy Vector Kit (Promega), which has a plasmid of 3015 bp as cloning vector and a cleavage site for each of the restriction enzymes used in the analysis (in the linker), including two EcoRI sites flanking the PCR product cloning site.
The purified PCR product was ligated with the pDrive cloning vector and then transformed into the QIAGEN EZ competent cell.
The amplified product was purified by PCR purification kit following manufacturer's instructions (Qiagen, Germany) and cloned first in the TA cloning vector pTZ57R (Fermantas) followed by subcloning in the yeast shuttle expression vector pGal426 that contains the galactose inducible promoter Gall and uracil synthesizing gene (ura3) as selection marker (12,13).
pSPPO1 contained a BamHI fragment and pAPPO1 a SacI fragment of cloning vector between the promoter and the sense transgene, and between the antisense transgene and terminator respectively.
The eluted product was cloned into pTZ57R/T cloning vector using Ins T/A clone PCR product cloning kit [MBI, Fermentas Life Sciences, USA (#K1214)] after determining the appropriate vector: insert ratios (Sambrook et al., 1989).
Then, the DNA fragments were ligated into the cloning vector pUC118 which had been previously digested by BamHI and dephosphorylated with calf alkaline phosphatase (TaKaRa, Dalian).
The DNA cloning vector pcDNA3.1 (Invitrogen, USA) for the construction of recombinant plasmids and baby hamster kidney (BHK-21) cells were used for eukaryotic expression of the protein.