The molar ratio of secreted proBNP 1-76 to intact proBNP is not yet fully clarified but is likely to depend on cardiac status, i.e., more unprocessed precursor compared to biosynthetic cleavage products
in severe heart failure.
When reexamined, three of the samples did contain weak cleavage products
corresponding to the expected fragment sizes when compared with cDNA sequence data but were not registered as mutated during the blind phase of the study.
In samples with wild-type CBS, heteroduplexes are not formed, and as expected, there was no cleavage product
. In samples containing two different alleles, hydroxylamine treatment produced distinct peaks of the size expected for both mutations.
A [sup.32]P-labeled marker oligonucleotide was used as a size marker for the cleavage products
. The radioactivities of the intact and cleaved oligonucleotides were quantified using an FLA-3000 fluoro image analyzer (Fuji Film, Tokyo, Japan) and Image Gauge software (Fuji Film).
The cleavage products
were visualized by agarose gel (2%).
The Peptide Synthesis section offers information on solid phase peptide synthesis and coupling method, and resin cleavage products
and their colorimetric tests are explained.
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Although BACE1 and BACE2 have the same biological activity against the APP substrate, their cleavage sites on APP are different, producing different cleavage products
which are amyloidogenic for BACE1 and nonamyloidogenic for BACE2.
Brennan, "Preparation of recombinant thioredoxin fused N-terminal proCNP: analysis of enterokinase cleavage products
reveals new enterokinase cleavage sites," Protein Expression and Purification, vol.
The enzyme cleaves at the site of the mismatch and cleavage products
are detectable and indicative of the presence of a potential mutation in a target DNA sequence.
[sz]-Amyloid (A[sz]) peptides are generated as cleavage products
39 to 43 amino acids in length from the membrane protein, Amyloid Precursor Protein (APP) by two proteases, ?-secretase and ?-secretase.1-3 While only a small amount is processed by ?-Secretase, also known as BACE1 (?-secretase APP cleaving enzyme) or memapsin, APP is predominantly processed by ?-Secretase, producing a 83-amino acid C-terminal fragment, C83.