An oligopeptide that is known to inhibit chymotrypsin-like proteases (for example, cathepsin A, B, and D, and papain).
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4) containing 1 mM EDTA, 1 mM dithiothreitol, 5 mg/L phenylmethylsulfonyl fluoride, 5 mg/L aprotinin, 5 mg/L chymostatin, and 5 mg/L pepstatin added phosphatase inhibitor, sodium orthovanadate to a final concentration of 2 mM.
Diquat (dibromide monohydrate, West Chester, PA), D-mannitol, HEPES, dithiothreitol, EDTA, phenylmethyl-sulfonyl fluoride, aprotinin, chymostatin, pepstatin A, Triton X-100, L-ornithine, pyridoxal phosphate, [alpha]-ketoglutarate, o-aminobenzaldehyde, o-phthaldialdehyde, L-arginine, L-citrulline (Sigma, Buchs, Italy) were procured from Sigma (St.
4, protease inhibitors (5 mg/L phenylmethyl-sulfonyl fluoride, 5 mg/L aprotinin, 5 mg/L chymostatin and 5 mg/L pepstatin A)).