Formaldehyde in alcoholic beverages: Large chemical survey using purpald screening followed by chromotropic
acid spectrophotometry with multivariate curve resolution, International Journal of Analytical Chemistry, 2011, Article ID 797604.
The method used colorimeter, the principle of which is formaldehyde reacting with chromotropic
acid-sulfuric solution to form a purple monocationic chromogen and being read by spectrophotometer at 580 nm.
Lots of the reported methods have relied on derivatizing formaldehyde using acetylacetone or chromotropic
acid reagents prior to colorimetric and/or spectrophotometric determination.
The precipitate was dissolved in 5 ml of chromotropic
reagent and vigorously shaken, and the tube was then placed in a boiling water bath for 30 min.
The reaction system consisted of the lens supernatant, 12.5 mM periodic acid, 12.5 mM sodium arsenite, and 0.2% chromotropic
Total amount of P (PO 3--P) in the samples was determined by Molybdate-Vanadate reagent method and N (NO--N) was analyzed by chromotropic
acid methods (Kowalenko and Lowe, 1973).
Uptical density was determined at 570 nm after formation of a colored complex of formaldehyde and chromotropic
In the next step, 5.0 ml chromotropic
acid reagent was added.
Method based on reaction of formaldehyde with chromotropic
acid and determination of formaldehyde concentration.
Subsequently, the sections were incubated with chromotropic
acid for 40 min at room temperature, and washed with distilled water for 10 s.