Extrinsic tooth staining can be caused by compounds that are incorporated into the pellicle and produce a stain consequently to their basic color (chromogen
), or those that lead to staining caused by chemical interaction at the tooth surface, which is usually associated with cationic antiseptics and metal salts.
Biotin would bond with horseradish peroxide enzymes and DAB chromogen
and forms brown-colored complex at that bond.
The amount of sandwiched histidine proteins was proportional to the color intensity following the addition of a chromogen
substrate into the ELISA wells.
Fe[Cl.sub.3] produce CCI only with (-OH) chalcones, while NaB[H.sub.4]-HCl and [Ac.sub.2]O-[H.sub.2]S[O.sub.4] reagents indicate that the chromogen
involved in formation CCI of chalcone is (Ar-CO-CH=CH-Ar).
Protein expression was visualized using diaminobenzidine as chromogen
. The relative gray values of corresponding bands and [beta]-actin were then semi-quantified and compared by Scion Image analysis software.
TEAC is a spectrophotometric technique that measures the relative ability of hydrogen-donating antioxidants to scavenge the 2, 2'-azino-bis3-ethylbenzthiazoline-6-sulfonic (ABTS+) radical cation chromogen
in relation to that of Trolox, the water soluble vitamin E analogue, which is used as an antioxidant standard.
In addition, Harlequin Listeria Chromogenic Agar uses a novel chromogen
to distinguish L.
This dual chromogen
medium enables the isolation of salmonella (including Salmonella typhi and S.
was added to the sections for 5 minutes to give color to the antigens and sections were again washed with TBS.
The immunoperoxidase stain used was a commercial rabbit polyclonal antibody (BioRelience Corp., Rockville, MD, USA) with peroxidase-tagged goat antirabbit immunoglobulin G (DakoCytomation, Carpinteria, CA, USA) bridge and 3-amino-9-ethylcarbazole (DakoCytomation) as the chromogen
. This staining demonstrated abundant WNV antigen within the cytoplasm of a small number of neurons and glial cells and in fewer macrophages in the brain tissue (Figure).
Briefly, 20 ul of serum sample (or standard, 6-hydroxy-2,5,7,8- tetramethylchroman-2-carboxylic acid) was incubated with 1 ml of chromogen
(consisting of metmyoglobin and ABTS(r)) at 37degC in a cuvette in temperature-controlled spectrophotometer.
In the Morgan-Elson reaction the NAG in the reducing end is successfully transformed into a chromogen
under alkaline condition at 100[degrees] C and subsequently by the action of p-dimethyl amino benzaldehyde (p-DMAB) in glacial acetic acid and hydrochloric acid mixture (9:1; v/v) to give a reddish purple colored product that can be detected at 585 nm.