Unfortunately, isomeric [gamma]-halo-[delta]-lactones 3,5,7, the typical principal products of the reaction, were not divided chromatographically
into enantiomers, even at retention times of more than 60 minutes.
In the second phase, [C.sub.60], [C.sub.70], and the higher fullerenes [C.sub.76] and [C.sub.84] (the only stable [C.sub.60]-Ih, [C.sub.70]-D5h, and [C.sub.76]-[D.sub.2] isomers of the first three mentioned fullerenes and the most abundant, stable [C.sub.84] isomer of [D.sub.2] symmetry) were chromatographically
separated from the obtained extracts of the carbon soot on the activated [Al.sub.2][O.sub.3] columns by new and advanced methods [2, 3, 38-45].
The recombinant HIV-1/2 antigen (gp24, p41 and gp36) gold conjugate and the sample move chromatographically
to the test region (T) and develop a visible line as the antigen--antibody--antigen gold particle complex with a high degree of specificity and sensitivity.
Methods: The mobile phase composed of acetonitrile-water 60:40 (v/v) and 0.2% formic acid 20 [micro]l of sample was chromatographically
analyzed using a repacked ZORBAX-XDB-ODS [C.sub.18] column (2.1 mmx30 mm, 3.5 [micro]).
This work also focused on the chlorophenol analysis of three tap water samples, comparing them both chromatographically
The mixture migrates upward on the membrane chromatographically
by capillary action to react with the recombinant antigen on the membrane and generate a coloured line.
Since these lists include isobaric compounds that must be separated chromatographically
, column choice is a critical decision.
Currently this chromatographically
purified Vi antigen (cp-Vi) is used in the production of typhoid vaccine (14).
Three subsequent condensation steps were proceeded out at specific conditions of temperature and pH with the help of stirrer and monitored chromatographically
. Diazotization and then coupling was accomplished by adding respective reactive acids (H-acid) 1- amino- 8- hydroxynapthalene- 3, 6 disulphonic acid to the reaction mixture.
Thirty microliter of the resulting amino acid solution were injected into an amino acid analyzer "Biochrom 30 plus" (Onken, Griindau, Germany), where the amino acids were separated chromatographically
, derivatized with ninhydrin, and absorption measured at wave lengths 440 and 570 nm.
Working with biologists and the firm Air Variable, the artist embarked on a process of conversion: she chromatographically
analyzed scent molecules from air samples of the collected bacteria, as well as air samples taken at Gagosian Gallery in New York during the run of an Urs Fischer exhibition.