In this connection, the study investigated the antihyperlipidemic potential (mediated via impairment of lipid synthesis, digestion, and absorption) of alba grade bark of true cinnamon by assessing its inhibitory activities on HMG-CoA reductase, lipase, cholesterol esterase
, and cholesterol micellization and effect on bile acid binding in vitro.
To quantify total amount of cholesterol in U937 macrophages, we used a colorimetric method in the presence of cholesterol oxidase and cholesterol esterase
. Exposure for 5 days to 10 [micro]g/[cm.sup.2] DEP as well as to UDP stimulated the accumulation of cholesterol by 2-and 2.5-fold, respectively (Figure 8).
Tsang and his coworkers exposed three commonly used methacrylate polymers to buffer solutions both with and without cholesterol esterase
for periods of 2 to 8 days.
LDL, VLDL and chylomicrons react with PVS and PEGME and that results in inaccessibility of LDL, VLDL and chylomicron to cholesterol oxidase and cholesterol esterase
, whereas HDL reacts with the enzymes.
Reagent 1 consisted of 600 U/L cholesterol esterase
, 500 U/L cholesterol oxidase, 1200 KU/L catalase, 2 mmol/L N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline, sodium salt, and dihydrate in PIPES buffer (50 mmol/L, pH 7.0).
Cholesterol oxidase, cholesterol esterase
, peroxidase and 25% glutaraldehyde were purchased from M/s Fluka Chemicals Germany.
 Nonstandard abbreviations: TRL, triglyceride-rich lipoprotein; CAD, coronary artery disease; VLDLR, VLDL remnant; IDL, intermediate-density hpoprotein; RLP-C, remnant-like particle cholesterol; apo, apolipoprotein; CM, chylomicron; LPL, lipoprotein lipase; CMR, CM remnant; TG, triglyceride; PL-D, phospholipase D; CHER, cholesterol esterase
; CHOD, cholesterol oxidase; POE-POB, polyoxyethylene-polyoxybutylene block copolymer.
Serum Total cholesterol (TC) was estimated by cholesterol esterase
method and High Density Lipoprotein (HDL) by cholesterol esterase
method after precipitation using phosphotungstate method.
On the other hand, BuChE may play a role in lipid metabolism (21), whether directly (19) or through a synergistic action with cholesterol esterase
, as has been demonstrated in saliva (22).
This method uses a detergent polymer mixture (reagent 1) to release cholesterol from non-LDL lipoproteins that reacts with cholesterol esterase
, cholesterol oxidase, peroxidase, and 4-aminoantipyrene to produce colorless products.
This assay is derived from the specific detection of cholesterol in HDL particles, achieved by polyethylene glycol-modified cholesterol esterase
and cholesterol oxidase and masking of other lipoprotein particles with sulfated cyclodextrin.
Agarose electrophoresis was substantially improved by the introduction of enzymatic cholesterol determination using cholesterol esterase
, cholesterol dehydrogenase, and nitroblue tetrazolium chloride dye (32-34) (Table 1).