cholesterol esterase

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Related to cholesterol esterase: Cholesteryl esterase

cholesterol esterase

/cho·les·ter·ol es·ter·ase/ (kah-les´ter-ol es´ter-ās) acid lipase; an enzyme that catalyzes the hydrolytic cleavage of cholesterol and other sterol esters and triglycerides. Deficiency of the lysosomal enzyme causes the allelic disorders Wolman's disease and cholesteryl ester storage disease.


A gene on chromosome 10q23.2 that encodes lipase A, the lysosomal acid lipase (i.e., cholesterol ester hydrolase) that catalyses the hydrolysis of cholesteryl esters and triglycerides in lysosomes.

Molecular pathology
LIPA mutations are linked to CESD and Wolman disease.
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Cholesterol oxidase, cholesterol esterase, peroxidase and 25% glutaraldehyde were purchased from M/s Fluka Chemicals Germany.
Mutual influence of cholesterol esterase and pseudocholinesterase on the biodegradation of dental composites.
Cholesterol from non-LDL lipoproteins reacts with cholesterol esterase, cholesterol oxidase, catalase, and N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline to produce colorless products.
Recently, an electrophoretic method using cholesterol esterase and cholesterol oxidase with aminoethyl carbazole dye was reported (38).
2+] were found to selectively block but not precipitate chylomicrons and VLDL, providing selectivity without the need for a clearing reagent; and (b) the specificities of the enzymes cholesterol esterase and cholesterol oxidase toward HDL-C were enhanced by covalently linking polyethylene glycol molecules to the enzymes, excluding cholesterol in the larger LDL particles.
The enzymatic reagent for cholesterol contained cholesterol esterase, cholesterol oxidase, peroxidase, phenol, and 4-amino antipyrine in PIPES buffer (4) and was purchased from RANDOX.
After the addition of cholesterol esterase and cholesterol oxidase (step 1B), only cholesterol from HDL particles was detected, because the enzymes were sterically blocked by the antibody from reacting with cholesterol on the apoB-containing lipoproteins.
The solubilized cholesterol is consumed in a non-color-producing reaction by cholesterol esterase and cholesterol oxidase.
Non-LDL lipoproteins are disrupted by a detergent, and the released cholesterol is hydrolyzed by cholesterol esterase.
They involved releasing the undesirable lipoprotein components by means of several components included in reagent 1 (selective ionic strength buffers, cholesterol esterase, cholesterol oxidase, and catalase).
Automated agarose gel electrophoresis of lipoproteins in alkaline buffer was adapted to allow the direct quantification of cholesterol in each lipoprotein fraction within the gel, using the cholesterol esterase /cholesterol oxidase reaction (SEBIA).
The reaction principle of Accutrend Triglycerides is lipolysis of triglycerides to give free glycerol and fatty acids by the lipase activity of a cholesterol esterase.

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