They grow on blood agar and often on chocolate agar
, but not on MacConkey or heart infusion agars .
These were then inoculated on Blood, MacConkey and Chocolate agar
and incubated at 37degC for 24-48 hours.
The growth from chocolate agar
and BHI agar was also sent for cytological study, which also confirmed the presence of earlier-mentioned microscopic picture.
Nutrient agar was used to isolate non fastidious organisms; chocolate agar
was for isolating fastidious organisms, while MacConkey agar was used to differentiate between lactose and non-lactose fermenting organisms.
The aspirate was inoculated on to blood and chocolate agar
(CO2 incubation at 37[degrees]C) and Fastidious Anaerobe Agar (anaerobic incubation at 37[degrees]C).
661 patients met the criteria; their age, sex, levels of CSF glucose, protein, white blood cell (wbc) count and reports of CSF culture in chocolate agar
media at admission as well as their prognosis on 15 day follow up based on extended Glasgow outcome score (GOS-E) were documented.
Two sets of blood agar and chocolate agar
plates were prepared and were tested for the growth of Acanthamoeba with autoclaved and live E.
These organisms are facultative anaerobic and grow on routine laboratory media such as blood (BAP), chocolate agar
plate (CAP), colistinnalidixic acid (CAN), and phenyl ethyl alcohol (PEA) agars.
Serial dilutions were performed and plated on chocolate agar
. Plates were incubated at 30 [degrees]C for 48 h, after which CFU/ml was determined via plate counting.
The pus was plated on chocolate agar
and MacConkey's agar.
The identity of the organism was confirmed by specific characteristics after culture on chocolate agar
supplemented with PolyVitex plus colistin, vancomycin, amphotericin B, and trimethoprim antibiotics (bioMerieux SA).
Specimens were taken from the transport vial with a 0.01-ml calibrated loop and inoculated into a Columbia agar base supplemented with 5% sheep's blood, chocolate agar
, eosin-methylene-blue agar, and Chapman agar (for aerobic and facultative bacteria) and onto a Brucella agar base supplemented with 5% sheep's blood, vitamin [K.sub.1], hemin with and without kanamycin, and thioglycolate medium without an indicator and supplemented with vitamin [K.sub.1] and heroin (for anaerobic bacteria).