Chitinolysis is performed by three separate enzymes namely endochitinases which produce multimers of N-acetlyglucosamine (NAG), exochitinases which produce low molecular weight chitobiose
that are subsequently hydrolyzed to NAG by chitobiases (8).
Kim, "High antioxidant and DNA protection activities of N-acetylglucosamine (GlcNAc) and chitobiose
produced by exolytic chitinase from Bacillus cereus EW5," Springer Plus, vol.
Furthermore, it was noticed for marine bacteria that glucose, trehalose and chitin hydrolysis end products e.g., N-acetyl glucosamine, glucosamine and chitobiose
play an important role in chemotaxis and induce the chitinase production [37,38,39].
The synthesis of chitoheptaose has been achieved by using chitopentaose oxazoline as glycosyl donor and chitobiose
as glycosyl acceptor catalyzed by a mutant chitinase.
Indeed, TLC and MALDI-TOF analyses identified chitobiose
as the single MetaChi18A product of [beta]-chitin and the main product of colloidal chitin hydrolysis.
found that, among the five tested chitooligosaccharides (chitobiose
to chitohexaose), chitohexaose had the most potent inhibitory effect on proliferation of the human lung carcinoma cell A549 .
As the second-most abundant biopolymer after cellulose, chitin is mainly synthesized via a biosynthetic process by an enormous number of living organisms such as shrimp, crab, tortoise, and insects and can also be synthesized by a nonbiosynthetic pathway through chitinase-catalyzed polymerization of a chitobiose
fischeri then migrate through the pores in response to a chitobiose
gradient to which they undergo chemotaxis (Mandel et al., 2012; Kremer et al, 2013).
In the presence of the inhibitor (chitobiose
), these height fluctuations decreased to the level without the polyglycoside.
The average molecular weight of COS was 1,500 Da and the major components of COS were chitobiose
, chitotriose, chitotetraose, chitopentaose and chitohexaose (Li et al., 2007).
Chitin is the organic skeletal substance of the shells of crustaceans and may be considered to be a linear polymer of chitobiose
, an aminopolysaccharide with a molecular weight of several hundred thousand.
Cloning and expression of chitinase A from Serratia marcescens for large-scale preparation of N, N diacetyl chitobiose