Clear zone diameters are measured (mm) and used to indicate the chitinase
activity of each isolate.
The following values of enzymatic activity were observed: 530 [+ or -] 46 U/mL for proteases and 0 for chitinases
. Thus, although several plant latex exhibit high amounts of chitinase
, in the case of the present S.
Proteomic analysis reveals suppression of bark chitinases
and proteinase inhibitors in Citrus plants affected by the Citrus sudden-death disease.
activity, P aeruginosa PAO1 was added with sodium citrate buffer (0.1 M, pH 4.8) containing chitin azure (0.5 mg/ml).
isolates produced pectinases and cellulases, but not chitinase
and [beta] 1,3 glucanase.
3-like 1: prognostic biomarker in clinically isolated syndromes.
There was no significant difference in phosphatase activity between the CK and 26ML treatments, but activities of [beta]-glucosidase and chitinase
were significantly greater in the CK than the 26ML treatment (Fig.
Necrotrophic fungal plant pathogens display different mechanisms to counteract grape chitinase
and thaumatin-like protein.
In contrast, none of the test strains exhibited chitinase
activity (Table 6).
Activities of chitinase
(CHI) and [beta]-1,3-glucanase (GLU) were determined as previously described in .
tuberculatum venom can be grouped basically into proteins involved in insect metabolism (annexin B9, nucleoside diphosphate kinase; aconitate hydratase; superoxide dismutase; triose phosphate isomerase; glyceraldehyde-3phosphate dehydrogenase; succinyl-CoA ligase; fructosebisphosphate aldolase; arginine kinase; citrate synthase; enolase; ATP synthase; 4-hydroxybutyrate coenzyme A transferase; medium-chain specific acyl-CoA dehydrogenase, and isocitrate dehydrogenase); transport protein (transferrin); and structural proteins (cyclophilin, actin, and chitinase
Of the 102 cytokines assessed, the array showed increased expression of 21 proteins in the cocultured samples: adiponectin, chitinase
3-like 1, complement factor D, CXCL5, endoglin/CD105, IGFBP-3, IL-4, IL-6, IL-16, IL-23, IL-24, IL-33, leptin, LIF, myeloperoxidase, osteopontin, pentraxin-3, CCL5/RANTES, serpinE1, CCL17/TARC, and uPAR.