chimerism analysis

chimerism analysis

The analysis of haematopoietic chimerism after allogeneic bone marrow (BM) transplantation, which allows detection of early BM engraftment, disease relapse or graft rejection.
 
Techniques
RFLP by Southern blotting, variable number of tandem repeats (VNTRs) by PCR.
References in periodicals archive ?
Here, we demonstrate single-molecule molecular inversion probe (smMIP) capture (19, 20) coupled with high-throughput sequencing to identify and quantify deletion copy number variants for the purposes of chimerism analysis (Fig.
Chimerism analysis by polymerase chain reaction of short tandem repeats (STR-PCR) showed full-donor chimerism in all lineages, which confirmed the donor-cell origin for the MDS.
Other antibodies used for chimerism analysis including CD45, CD4, CD8, CD11b, CD11c, CD19, Ly6G/6C (Gr1), and Foxp3 were purchased from BD Biosciences (San Jose, CA, USA), eBioscience Inc., (San Diego, CA, USA), and BioLegend Inc., (San Diego, CA, USA).
Chimerism analysis was performed by DNA fingerprinting for short-tandem repeats in blood samples and/or chromosome fluorescence in situ hybridization of BM samples [18].
Chimerism analysis on the peripheral blood showed 0% donor DNA.
Chimerism analysis is an important tool to assess the origin of hematopoietic cells after SCT.
Confirmation of donor engraftment was made either by cytogenetics for sex-mismatched transplants or by chimerism analysis of flow-sorted peripheral blood T cells and nucleated marrow cells.
Chimerism Analysis. The evidence of chimerism was observed by means of sequence-specific primer amplification (SSP; Olerup, Sweden; data not shown).
Chimerism analysis using polymerase chain reaction (PCR)-based amplification of short tandem repeat (STR) markers has grown to be the most widely used tool for analyzing stem cell engraftment over fluorescent/ns/fu hybridization (FISH) and other cytological methods.
Catastrophic graft-versus-host disease after lung transplantation proven by PCR-based chimerism analysis. Transpl Int.