chimerism analysis

chimerism analysis

The analysis of haematopoietic chimerism after allogeneic bone marrow (BM) transplantation, which allows detection of early BM engraftment, disease relapse or graft rejection.
 
Techniques
RFLP by Southern blotting, variable number of tandem repeats (VNTRs) by PCR.
References in periodicals archive ?
Chimerism analysis in peripheral blood using indel quantitative real-time PCR is a useful tool to predict post-transplant relapse in acute leukemia.
Chimerism analysis using polymerase chain reaction (PCR)-based amplification of short tandem repeat (STR) markers has grown to be the most widely used tool for analyzing stem cell engraftment over fluorescent/ns/fu hybridization (FISH) and other cytological methods.
Catastrophic graft-versus-host disease after lung transplantation proven by PCR-based chimerism analysis.
In a research context, plasma DNA chimerism analysis promises new avenues for monitoring the immunological rejection of organ transplants.
PERFORMANCE OF CND qPCR ASSAYS FOR CHIMERISM ANALYSIS
Patients who were transplanted before 2001 had their chimerism analysis done only after 2001 at whichever postHSCT date they were at.
Chimerism analysis within 6 months of allogeneic stem cell transplantation predicts relapse in acute myeloid leukemia.
In chimerism analysis, quantification is of importance when examining patients who have received minitransplants because the number of donor cells is crucial and must be quantified in the patient's blood (which still contains high numbers of recipient cells indistinguishable by their phenotype) (6).