chimerism analysis

chimerism analysis

The analysis of haematopoietic chimerism after allogeneic bone marrow (BM) transplantation, which allows detection of early BM engraftment, disease relapse or graft rejection.
 
Techniques
RFLP by Southern blotting, variable number of tandem repeats (VNTRs) by PCR.
References in periodicals archive ?
In a research context, plasma DNA chimerism analysis promises new avenues for monitoring the immunological rejection of organ transplants.
PERFORMANCE OF CND qPCR ASSAYS FOR CHIMERISM ANALYSIS
After working with collaborators throughout the world, we are excited to see such positive results from complex applications, like 12-color hematopoietic cell transplantation chimerism analysis, 11-color lymphocyte lymph-node replacement kinetics, and 7-color pediatric leukemia signal transduction," says Dr.
Chimerism analysis using polymerase chain reaction (PCR)-based amplification of short tandem repeat (STR) markers has grown to be the most widely used tool for analyzing stem cell engraftment over fluorescent/ns/fu hybridization (FISH) and other cytological methods.
Catastrophic graft-versus-host disease after lung transplantation proven by PCR-based chimerism analysis.
They include assessment of donor hematopoietic engraftment after bone marrow transplantation and chimerism analysis after solid organ transplantation.
The STR assay is also useful in chimerism analysis after liver transplantation.
Chimerism analysis after bone marrow or stem cell transplantation offers an objective, quantitative measure for monitoring engraftment of donor cell populations in the new host.
In chimerism analysis, quantification is of importance when examining patients who have received minitransplants because the number of donor cells is crucial and must be quantified in the patient's blood (which still contains high numbers of recipient cells indistinguishable by their phenotype) (6).