Here, we demonstrate single-molecule molecular inversion probe (smMIP) capture (19, 20) coupled with high-throughput sequencing to identify and quantify deletion copy number variants for the purposes of chimerism analysis
. The proportion of chimeric DNA in patients' MSCs was determined at passages 1-3.
by polymerase chain reaction of short tandem repeats (STR-PCR) showed full-donor chimerism in all lineages, which confirmed the donor-cell origin for the MDS.
Other antibodies used for chimerism analysis
including CD45, CD4, CD8, CD11b, CD11c, CD19, Ly6G/6C (Gr1), and Foxp3 were purchased from BD Biosciences (San Jose, CA, USA), eBioscience Inc., (San Diego, CA, USA), and BioLegend Inc., (San Diego, CA, USA).
was performed by DNA fingerprinting for short-tandem repeats in blood samples and/or chromosome fluorescence in situ hybridization of BM samples .
on the peripheral blood showed 0% donor DNA.
is an important tool to assess the origin of hematopoietic cells after SCT.
Confirmation of donor engraftment was made either by cytogenetics for sex-mismatched transplants or by chimerism analysis
of flow-sorted peripheral blood T cells and nucleated marrow cells.
. The evidence of chimerism was observed by means of sequence-specific primer amplification (SSP; Olerup, Sweden; data not shown).
using polymerase chain reaction (PCR)-based amplification of short tandem repeat (STR) markers has grown to be the most widely used tool for analyzing stem cell engraftment over fluorescent/ns/fu hybridization (FISH) and other cytological methods.
Myeloid cell chimerism analysis
performed 6 months post transplantation showed 99% donor chimerism, according to 6 alleles (ABI 310 genetic analyzer).
Catastrophic graft-versus-host disease after lung transplantation proven by PCR-based chimerism analysis
. Transpl Int.