The corresponding EML4ALK chimeric protein
demonstrated constitutive tyrosine kinase activity that conferred transformation potential and responsiveness to specific targeted inhibitors in vitro and in vivo.
Expression of the chimeric protein
was induced at [OD.
Expression of a chimeric protein
containing the catalytic domain of shiga-like toxin and human granulocyte macrophage colony-stimulating factor (Hgm-csf) IN Escherichia coli and its recognition by reciprocal antibodies.
Recent advances in molecular methodologies have allowed gene fusions and chimeric protein
tuberculosis H37Rv was over-expressed as a chimeric protein
(glutathione-S-transferase-PknG, GST-PknG) in E.
The ETV6-NTRK3 gene fusion encodes a chimeric protein
tyrosine kinase that transforms NIH3T3 cells.
The chimeric protein
contains three polypeptidyl fragments: (a) a first polypeptidyl fragment at the N-terminal end of the chimeric protein
that contains a protein transduction domain (PTD) or a fragment thereof having HIV Tat PTD activity; (b) a second polypeptidyl fragment at the C-terminal end of the first polypeptidyl fragment that contains a J-domain or a fragment thereof having heat shock protein 70 (Hsp70)-interacting activity; and (c) a third polypeptidyl fragment at the C-terminal end of the second polypeptidyl fragment that contains a target protein or polypeptide.
This chimeric protein
reveals what parts of GLUT4 play a role in indinavir's inhibition of glucose absorption.
ECI's hypothesis is that ECI characterized Protector Proteins alphaA-Crystallin, gammaD-Crystallin and the chimeric protein
, pepstatin /leupeptin/alphaA-Crystallin, will inhibit the proteolytic enzymes secreted by the host and the pathogen, thus serving as a good therapeutic agent against the management of microbial keratitis.
The high efficiency of protein expression obtained with this particular bgh-polyA signal became apparent as we subsequently documented its efficiency in a wide range of chimeric protein
expression systems, both in vivo and in vitro.
Although most of the autoreactive epitopes of GAD65 are located within the middle and the C-terminal regions of GAD65 (amino acids 245-585) (27, 34), truncated GAD65 lost almost all of its autoreactive epitopes when it was integrated in a chimeric protein
with IA2c (Fig.
This gene fusion is postulated to produce an oncogenic chimeric protein
with the zinc finger DNA-binding domains of WT1 with the transcriptional regulatory domain of the Ewing sarcoma gene.