Regression analysis was done as the appropriate statistical method to observe the dependence of various parameters, such as number and diameter of chief cells
and oxyphil cells with age.
(a), (d), (g), (j), and (m) epithelial cells; (b), (e), (h), (k), and (n) parietal cells; (c), (f), (i), (l), and (o) chief cells
. For statistical analysis, a nonparametric Kruskal-Wallis test was used.
Photomicrograph showing chief cells
, which are nonimmunoreactive for CAM 5.2 (original magnification X20).
A few of the endocrine cells distributed in the gastric glands, with round, conical or triangular in shape, which were mainly embedded in the chief cells
. The cytoplasm of the endocrine cells were filled with tiny argyrophilic granules so that we could observe at the neck and body of glands with Gordon-Sweet's staining.
Present study revealed that oxyphil cells were less numerous than chief cells
, placed either at periphery of gland adjacent to capsule or in the centre of gland.
Mills, graduate student Greg Sibbel and Hans Clevers, a geneticist at Utrecht Medical Center, identified markers that show a small number of chief cells
become stem cells even in the absence of serious injury.
C, Multiphoton microscopy reveals individual gastric oxyntic glands that are separated by a mesh of collagen fibers at the deeper part of the mucosal layer ([greater than or equal to] 60 m depth) D, The corresponding light-microscopic image shows there are chief cells
and parietal cells in each gastric gland (hematoxylin-eosin, original magnifications x400 [B and D]).
MUC6 is expressed in the cytoplasm of antral pyloric glands but also in mucous neck cells and chief cells
of the gastric body.
Histologically, these tumors appear highly vascular and are composed of clusters (Zellballen, which is German for "cell balls") of chief cells
supported by sustentacular cells and small blood vessels.
Histologically, the tumor was composed of uniform small round cells (chief cells
) arranged in discrete cohesive lobules (zellballen).
Histopathologic examination revealed that parts of the tumor contained a lobulated architecture composed of sheets, cords, and follicles of chief cells
, oxyphil cells, and clear cells; these findings were consistent with an adenoma.
Finally, mixtures of different cells (oxyphils, clear cells, and chief cells
) can be found in adenomas, as well as in hyperplastic glands. The most important criterion is the clinical follow-up--no recurrence of hypercalcemia on long-term follow-up (at least 5 years) supports the diagnosis of adenoma.