chaotropic agent


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chaotropic agent

An ion that disrupts membranes, nucleic acids, and proteins.
See also: agent
References in periodicals archive ?
Because previously reported chromatographic conditions (8,15,16) could not be applied directly to CSF analysis, we evaluated the optimum conditions by studying the effects of different salts, such as lithium, sodium, and potassium phosphate buffers; amines such as triethylamine (TEA), trimethylamine, morpholine and guanidine thiocyanate; and the chaotropic agent potassium perchlorate in different concentrations in mobile phase A.
The manual specimen preparation method isolates CMV DNA from 200 [micro]L of plasma by lysis of the virus with a chaotropic agent, guanidine thiocyanate, followed by precipitation of the DNA with isopropyl alcohol.
Given our observed inability to greatly alter soy flour adhesion using chaotropic agents, salts, surfactants, or co-solvents (Frihart and Lorenz 2013), which is in contrast to the literature observations with soy protein isolate (Sun 2005b), we concluded that the carbohydrates were suppressing protein alteration for improved adhesion.
Chaotropic agents are effective for removing nucleases, including RNases.
TEST PARAMETERS Inclusion Body Wash Solubilization Refold Buffer composition Buffer composition Buffer composition * Chaotropic agents * Chaotropic agents * Chaotropic agents * Detergents * Detergents * Redox agents * Salts * Reducing agents * Amino Acids * Detergents * Sugars * Salts w/v ratio Duration Duration Cycles of Protein Protein centrifugation concentration concentration pH w/v raio