After the samples were vortexed, they were subjected to centrifugation
at 2500 RPM for 5 minutes.
Analysis was done at time 0, 0.5, 1, 2, 4, 6 and 12th hour after drawing the samples, time zero was donated to the 1st sample which was analyzed after 30 minutes, standard time specified by the Vaccute to clot the sample properly before centrifugation
. Chemistry analysis was done on Selectra XL.
The lysis centrifugation
system showed less contamination as compared to conventional blood culture system (3.5% vs 6.8%).
The liquid-to-solid ratio 15, standing time 45 min, and centrifugation
time 6min were the conditions for cerebroside extraction.
The methods were centrifugation
and hydrothermal treatment applied after wet reaction.
To address this question, we examined the activated state of neutrophils when prepared by dextran sedimentation preceding density gradient centrifugation
with those prepared by steps which removed monocytes prior to the dextran sedimentation [13-15] or with another which totally avoided the use of dextran, the 1-step density gradient centrifugation
It is interesting to note that the value of [[lambda].sub.max] and the size of AuNPs powders made from spray drying and coagulation were almost not much changed in comparison with those of original AuNPs/dextran solution, but these parameters increased for the AuNPs made from centrifugation
The observation of multilayered cell sheets was performed by the OCT system on a hot plate (37[degrees]C, ThermoPlus) (Tokai Hit, Shizuoka, Japan) at 0, 15, 30, 45, and 60 min with/without centrifugation
. Those data were analyzed by the software ImageJ [21,22].
This shows that a centrifugation
step is required to obtain a suitable separation of the sample, but that a suitable speed has to selected, as if it is too great it will prevent the test from giving a true result.
rpm and increased time gave enhanced neutrophil granulocytes in the distal part (yellow color) of the clot
Percoll density-gradient centrifugation
method could be used to assess economic value and led to greater ease of use .
The company says with a wealth of engineering experience plus the equipment performance data and information that is only available to the original manufacturer, its optimisation team can identify potential improvements to equipment and systems, irrespective of the age of the centrifugation
equipment or process lines.