centrifugation


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Related to centrifugation: Differential centrifugation

centrifugation

 [sen-trif″u-ga´shun]
the process of separating lighter portions of a solution, mixture, or suspension from the heavier portions by centrifugal force.

cen·trif·u·ga·tion

(sen-trif'yū-gā'shŭn),
Subjection to sedimentation, by means of a centrifuge, of solids suspended in a fluid.
Synonym(s): centrifugalization

centrifugation

Lab technology The process of separating fractions of systems in a centrifuge. See Axial centrifugation, Density gradient centrifugation, Ultracentrifugation.

cen·trif·u·ga·tion

(sen-trif'ŭ-gā'shŭn)
Sedimentation, by means of a centrifuge, of solids suspended in a fluid. After the process, supernatant fluid and sediment are separated.

centrifugation

a process in which particles in suspension are subjected to centrifugal forces by being spun in a CENTRIFUGE, so forming a sediment. Subsequent removal of the SUPERNATANT fluid from the sediment and subjecting it to higher speeds of centrifugation can enable particles of different sizes to be separated out and collected. Eventually the supernatant fluid will contain only soluble materials. In some types of centrifugation, the sample is layered on to a column of liquid (such as sucrose) of increasing concentration down the column, and centrifuged until bands are formed. In this way the molecules are separated by their different rates of sedimentation. see DIFFERENTIAL CENTRIFUGATION, ULTRACENTRIFUGE.
References in periodicals archive ?
After the samples were vortexed, they were subjected to centrifugation at 2500 RPM for 5 minutes.
Analysis was done at time 0, 0.5, 1, 2, 4, 6 and 12th hour after drawing the samples, time zero was donated to the 1st sample which was analyzed after 30 minutes, standard time specified by the Vaccute to clot the sample properly before centrifugation. Chemistry analysis was done on Selectra XL.
The lysis centrifugation system showed less contamination as compared to conventional blood culture system (3.5% vs 6.8%).
The liquid-to-solid ratio 15, standing time 45 min, and centrifugation time 6min were the conditions for cerebroside extraction.
To address this question, we examined the activated state of neutrophils when prepared by dextran sedimentation preceding density gradient centrifugation with those prepared by steps which removed monocytes prior to the dextran sedimentation [13-15] or with another which totally avoided the use of dextran, the 1-step density gradient centrifugation method.
It is interesting to note that the value of [[lambda].sub.max] and the size of AuNPs powders made from spray drying and coagulation were almost not much changed in comparison with those of original AuNPs/dextran solution, but these parameters increased for the AuNPs made from centrifugation (Table 1).
The observation of multilayered cell sheets was performed by the OCT system on a hot plate (37[degrees]C, ThermoPlus) (Tokai Hit, Shizuoka, Japan) at 0, 15, 30, 45, and 60 min with/without centrifugation. Those data were analyzed by the software ImageJ [21,22].
This shows that a centrifugation step is required to obtain a suitable separation of the sample, but that a suitable speed has to selected, as if it is too great it will prevent the test from giving a true result.
Decreases centrifugation rpm and increased time gave enhanced neutrophil granulocytes in the distal part (yellow color) of the clot
Percoll density-gradient centrifugation method could be used to assess economic value and led to greater ease of use [27].
The company says with a wealth of engineering experience plus the equipment performance data and information that is only available to the original manufacturer, its optimisation team can identify potential improvements to equipment and systems, irrespective of the age of the centrifugation equipment or process lines.