The soluble and insoluble production of cellulase
was examined by activity assay and SDS-PAGE analysis (Laemmli, 1970).
The parameters used in the experimental design were pH (4-8, A), cassava pulp percentage (13%-20%, B), and cellulase
enzyme-concentration (0.1%-1% w/v, C) with a Box-Behnken design for three factors that enabled the construction of a second-order polynomial model to be used with Design Expert software version 7.15 (Stat-Ease, Inc.).
The IVDMD and IVOMD of 0.01% cellulase
treated silage at 6 and 48 h after incubation were greater (p<0.05) than those of 0.0025%.
Among the 38 bacterial isolates, 17 isolates showed a caseinase activity, 11 isolates showed amylase activity, 28 isolates showed esterase activity, 17 isolates showed cellulase
activity and 12 isolates identified as L-asparaginase producers as shown in table 1.
Purification and characterization of a protease-resistant cellulase
from Aspergillus niger.
To prepare a seed culture for ethanol fermentation, 1% (v/v) seed culture was inoculated in a medium containing enzymatic hydrolysis solution, in which 10% (w/v) pretreated soybean straw was hydrolyzed by the cellulase
for 48 h as carbon sources, 2% Bacto peptone, and 1% Bacto yeast extract as nutrient sources.
Conversely, cultures with cellulose or sophorose promoted the expression of MFS permeases at similar expression levels, including crt1 (ID 3405), required for cellulase
induction by cellulose and lactose, besides mediating the cellulose sensing process in T.
There were four kinds of enzymes in the experiment, named glucoamylase, cellulase
, phytase and pectinase.
, Trichoderma, viride, Substrates, Banana, Waste, Bagasse.
The data indicates that there was a large variation in cellulase
activity in the different assays carried out; this variation increases the importance of the experimental design used in this study because it may contribute to achieving higher enzyme production by adjusting the variables involved in SSF.
Screening of fermentation medium for cellulase
Supplementation of 2% OPFP treated with 20 U xylanase, 20 U cellulase
, and 10 U cellulase/10 U xylanase increased the EPS production by strain SLC 13.