MDSC Purified by CD48-Based
Cell Sorting Possessed Immune Suppressive Activity.
Finally, the inlet flow ratio [alpha] = [Q.sub.in1] : [Q.sub.in2] is also important to achieve the effective
cell sorting. To get a proper [alpha], a set of numerical cases are performed by setting [alpha] = 1/8, 1/6, 1/4, 1/2, 1, 2, 4, 6,8, and 10, where 20 cells with 8 [micro]m diameter(the smallest size) are initialized and randomly placed in the U-branch 2 to test the function of the pinched flow.
The authors thank Paz Reyes for technical help with
cell sorting and flow cytometry and Sebastian Valenzuela for valuable veterinary assistance at the Fundacion Ciencia & Vida animal facility.
Cultures of cells isolated from rat skeletal muscle were sorted using fluorescence-activated
cell sorting. Selection was based on the expression of an extracellular epitope of the muscle-specific [alpha]7 integrin protein (9 MBL International).
Our methods are useful in flow cytometry and fluorescence activated
cell sorting methods, microdissection methods, and for attracting, sedimenting, depositing, layering, attaching, adhering, bonding, binding, trapping or suspending cells on surfaces.
"We are enthusiastic about the opportunity to add Dako's MoFlo XDP high-performance sorter, the gold standard for
cell sorting, and the highly capable nine-color CyAn research flow cytometer to our existing portfolio of cytometry instruments," stated Scott Garrett, Beckman Coulter president and CEO.
BioDefense will evaluate the hepatic stem
cell sorting and enrichment technology, which can be applied to gene therapy and liver re-population technology, and will release more detailed information about the Hepatic Stem Cell technology and potential applications, including gene therapy and re-population in patients with liver disease, immediately after finalizing its evaluation and successful exercise of its option agreement by end of the 2nd Quarter of 2005.
Currently, there are two broad categories of enrichment methods: mechanical (centrifugation, cytospin, sucrose gradients, etc.) and antibody-based selection with mechanical separation (FACS--flow-assisted
cell sorting, MACS--magnetic assisted
cell sorting, etc.).
Harnessing the power of fluorescence-activated
cell sorting, the group was able to isolate specific root cells by introducing fluorescent proteins as a means to decipher location and, more importantly, to uncover which genes were expressed during corresponding phases of development.
In one example of success, a scientist was ruing to develop a macrophage assay by culturing macrophages and using
cell sorting. Normally he would have spent up to two weeks searching through literature to find examples of different protocols on various methods for culturing, and an additional two weeks to identify appropriate
cell sorting technology.
The scientists are preparing and concentrating pathogens using techniques such as sequential filtration, gravitation, immunotrapping, ligand-receptor interactions and
cell sorting. If a pre-enrichment step is necessary, they'll develop processes that shorten the incubation times required.
The eighth and ninth chapters provide good introductions to the topics of DNA analysis and flow cytometric
cell sorting.
