Alternative regimens: Spectinomycin (not available in the United States) 2 g in a single dose IM or cephalosporin single-dose regimens (including ceftizoxime
500 mg IM, or cefoxitin 2 g IM administered with probenecid 1 g orally, or cefotaxime 500 mg IM).
The routine antibiotic sensitivity tests were put up for aminoglycosides [amikacin (30 [micro]g), gentamicin (10 [micro]g), netilmicin (30 [micro]g), tobramicin (10 [micro]g)], cephalosporin's [cefoperazone (75 [micro]g), cefepime (30 [micro]g), ceftazidime (30 [micro]g), ceftriaxone (30 [micro]g), ceftizoxime
(30 [micro]g)], floroquinolones [ciprofloxacin (5 [micro]g), gatifloxacin (5 [micro]g), lomefloxacin (10 [micro]g)], carbapenems [imipenem (10 [micro]g), meropenem (10 [micro]g)], chloramphenicol (30 [micro]g) and piperacillin/tazobactum (100/10 [micro]g).
After 24-h incubation at 35[degrees]C, the enrichment broth was subcultured on Columbia agar plates with 5% sheep's blood (CA), a MRSA-ID plate, and 1 mL of the enrichment broth was put into a second enrichment broth containing phenol-red mannitol broth with ceftizoxime
(5 [micro]g/mL) and aztreonam (7.
Results: The isolates showed resistance to 13 different antibiotics ceftizoxime (99%), lomefloxacin (94.
Various antimicrobial agents ([micro]g) used were amikacin (30), gentamicin (10), tobramycin (10), ceftazidime (30), ceftriaxone (30), ceftizoxime (30), cefoperazone (75), carnbenicillin (100), piperacillin (100), Ticarcillin (75), ciprofloxacin (5), lomefloxacin (10) and imipenem (10) (Hi-media, Mumbai, India).
The resistance pattern to different antibiotics for the 104 isolates was as follows: ceftizoxime (99%), lomefloxacin (94.
The samples were plated on sheep blood agar and incubated in tryptic soy broth, 4% saline, 1% mannitol, phenol red (16 [micro]g/ mL), ceftizoxime
(5 [micro]g/mL), and aztreonam (50 [micro]g/mL).
Antimicrobial drug susceptibility was performed with broth microdilution according to the Sensititre user manual and National Committee for Clinical Laboratory Standards (NCCLS) guidelines (7) for amikacin, amoxicillin-clavulanic acid, ampicillin, cefazolin, ceftiofur, ceftizoxime
, chloramphenicol, enrofloxacin, gentamicin, tetracycline, ticarcillin-clavulanic acid, and trimethoprim-sulfamethoxazole.
The extended-spectrum cephalosporins--cefoxitin, cefotetan, cefotaxime, ceftizoxime
, and cefepime--are the most applicable to ob.