For confirmation of ESBL production ceftazidime (30ug), ceftazidime + clavulanic acid combination disc (30/10ug) manufactured by HiMedia and cefotaxime
(30ug) + cefotaxime
clavulanic acid (30/10ug) prepared in laboratory were used.
We found that 11 isolates were more resistant to cefotaxime
(MIC [greater than or equal to] 256 [micro]g/mL) than to ceftazidime (MIC [less than or equal to] 128 [micro]g/mL), which suggests CTX-M-type enzymes.
Of these strains, 14 (42%) of 33 were resistant to cefotaxime
Mineral oil 100% us gallon topical,1 gram cefotaxime
sodium vial powder for injection iv,dimenhydrinate 50mg / 2ml injectable solution iv.
All the specimens were inoculated on the MacConkey agar medium containing 2mg/L cefotaxime
After co-cultivation, cotyledonary node explants were transferred to selective medium (MS + 2 mg L-1 6-BA + 2 mg L-1 AgNO3 + 300 mg L-1 cefotaxime
+ 50 mg L-1 kanamycin).
The antibiotic susceptibility disks Aztreonam (30g), Cefotaxime
(30g), Ceftazidime (30g) and Ceftriaxone (30g) were placed on Mueller Hinton agar.
For bacteria isolated from diabetic foot infection, the following antibiotics were used against the causative agents: amikacin (30ug), (20ug of amoxicillin + 10ug of clavulanate), cefotaxime
(30ug), ceftazidime (30ug), ciprofloxacin (5ug), erythromycin (15ug), gentamycin (10ug), oxacillin (1ug), piperacillin (100ug) and vancomycin (30ug).
were showed 100% sensitivity to Cefotaxime
whereas complete resistance to Penicillin G and Amikacin were observed for Gram negative bacterial isolates.
Detection of extended spectrum beta lactamases was done by using Ceftazidime (30 [micro]g) and Ceftazidime (30 [micro]g)/Clavulanic acid (10 [micro]g) and Cefotaxime
(30[micro]) and Cefotaxime
(30 [micro]g)/Clavulanic acid (10 [micro]g).
Sensitivity of gram-negative and gram-positive organisms to cefotaxime
Since the first report from Spain in 2000 of strains producing CTX-M-9 (6), which confers resistance to cefotaxime
, various CTX-M-type ESBLs have been identified in Salmonella Virchow.