A total of 150 gynaecological surgeries were taken for the study, such that 75 patients were given cefazolin prophylaxis & the next 75 patients were given cefotaxime
coli isolates showed more sensitivity to Amikacin 57(76%) followed by Imipenem 57(66.67%), Ofloxacin 48(64%), Ciprofloxacin 44(58.67%), Norfloxacin 42(56%) and 75(100%) were resistant to Penicillin, Ceftriaxone, Ceftazidime and Cefotaxime
(Table-2 and Figure-2).
As no endovascular source was confirmed, it was decided that the patient would complete a 2-week course with ampicillin and cefotaxime
from the date of first negative culture.
The sludge samples (1 mL) were inoculated in 2 mL of Brain Heart Infusion (BHI) broth which contained cefotaxime
(2 mg/L) and then the sample was incubated at 37AdegC for two hours under aerobic conditions.
The highest rooting frequency (100.00%) was observed on MS medium supplemented with 0.2 mg L-1 indole acetic acid (IAA) and 400 mg L-1 cefotaxime
. The rooting frequencies observed on MS medium, supplemented with 0.2 mg L-1 a-naphthaleneacetic acid (NAA) and 400 mg L-1 cefotaxime
, and on 1/2 MS medium, supplemented with 0.2 mg L-1 IAA and 400 mg L-1 cefotaxime
, were 89.5% and 83.13%, respectively.
Phenotypic confirmatory test of provisional ESBLs producers was carried out by combined disk method by using antimicrobial disks of Ceftazidime (30g) and Cefotaxime
(30g) alone and in combination with Cla-vulanic acid (30/10g) on Mueller Hinton agar plates.
(Table 5) was found to be maximum sensitive for Ciprofloxacin (100%) followed by Norfloxacin (100%), Ceftriaxone (100%), Cefotaxime
(100%) and Tobramycin (50%) while resistant to Chloramphenicol, Gentamicin, Amikacin, Kanamycin, Neomycin, Enrofloxacin, Cephalexin, Clindamycin and Streptomycin.
aureus, cloxacillin (96.77%), cefotaxime
(91.94%), and ciprofloxacin (77.42%) were found to be the most effective drugs with the least resistance.
The rates of cefotaxime
resistance in Salmonella Virchow have increased markedly, from 21.4% in 2011 to 82.3% in 2014.
aeruginosa isolates were found to be sensitive to ceftazidime which is also supported by another study.18 On the other hand only 37% of isolates showed sensitivity to cefotaxime
in our study; meaning that in our set up, ceftazidim is effective but resistance against cefotaxime
has increased in the recent past.
sodium salt was purchased from Pharmaceutical Laboratory of Cuba (IMEFA).
Conclusion: Single dose antibiotic (cefotaxime
) is as effective as multiple doses of antibiotics (cefotaxime
) in the prophylaxis of postoperative wound infection in elective caesarian section.