caspase 9


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Related to caspase 9: Caspase 3, APAF3

caspase 9

A member of the CED-3 subfamily caspase-type protease activated by Apaf-1 binding, which is responsible for the proteolytic cleavage of many key proteins in apoptosis; caspase-9 plays a central role in cell death through an array of apoptosis inducers—e.g., TNF-alpha, TRAIL, anti-CD-95, FADD, TRADD4.
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In contrast to T47D cell lines, MCF7 cell lines with tiliroside of 50 [micro]g/ml occurred weak expression of Caspase 8 (Figure 6) moreover Caspase 9 was not expressed.
These results have a similar profile for Caspase 9, in which tiliroside activates Caspase 9 and doxorubicin did not activate it.
It was linear with the result that Caspase 9 is not activated by tiliroside on MCF7.
This is through the release of Cytochrome c from mitochondria is a central event in the death receptor-independent, "intrinsic," apoptotic pathway which facilitates activation by Caspase 9 of the effector Caspases [1], which subsequently activates Caspase 3, and ultimately leads to the apoptotic cell death.
8 and Caspase 9 colorimetric assay: R&D Systems, Inc.
California, USA) were used consisting of [beta]-Actin (1:1000), Bax (1:1000), Bcl2 (1:1000), Hsp70 (1:1000), survivin (1:1000), caspase 3 (1:1000) and caspase 9 (1:1000).
The influence of compound 3 on caspase 3/7, caspase 8 and caspase 9 activity in CCRF-CEM leukemia cells was detected using Caspase-Glo 3/7, Caspase-Glo 8 and Caspase-Glo 9 Assay kits (Promega, Germany).
5) and reacted with a primary polyclonal antibody, ASK-1, JNK, bcl-2, cyto c, caspase 9, caspase 3, PARP and [beta]-actin for 4h at room temperature.
Briefly, sections were blocked in diluted normal blocking serum for 20 min followed by 1 h incubation with primary rabbit polyclonal antibody ASK-1, JNK, cyto c, caspase 3, bcl-2, PARP (Cell Signaling Technology, Danvers, MA, USA), caspase 9 (Santa Cruz Biotechnology, Santa Cruz, CA, USA) and TUNEL (Roche Diagnostics, Laval, Q.
The influence of compounds 2-4 on caspase 3/7, caspase 8 and caspase 9 activity in CCRF-CEM leukemia cells was detected using Caspase-Glo 3/7, Caspase-Glo 8 and Caspase-Glo 9 Assay kits (Promega, Germany).
8 fold increase of caspase 9 activity, respectively (Fig.
Activation of caspase 9 and 8 occurred even at low concentration of PA, but caspase 3/7 activation occurred only at high concentration treatment.

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