This multi-component enzyme, which contains a biotin carboxylase activity, a biotin carboxyl carrier protein, and a carboxyltransferase
functionality, has major and minor subunits of [M.sub.r] 265,000 and 280,000 respectively [9-10].
The assay can be used for any enzyme class that produces ADP, including protein, lipid or carbohydrate kinases, ATPases, DNA helicases, and carboxyltransferases
. The "mix and read" product offers an excellent signal at low ATP conversion resulting in overall Z' >0.6.