carboxyltransferase

carboxyltransferase

 [kahr-bok″sil-trans´fer-ās]
an enzyme that catalyzes carboxylation.
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This multi-component enzyme, which contains a biotin carboxylase activity, a biotin carboxyl carrier protein, and a carboxyltransferase functionality, has major and minor subunits of [M.sub.r] 265,000 and 280,000 respectively [9-10].
The assay can be used for any enzyme class that produces ADP, including protein, lipid or carbohydrate kinases, ATPases, DNA helicases, and carboxyltransferases. The "mix and read" product offers an excellent signal at low ATP conversion resulting in overall Z' >0.6.