Further genetic analyses (i.e., candidate gene
studies) have helped identify specific genes that may contribute to the development of alcohol dependence and habitual smoking.
Geneticist Bruce Lahn of the University of Chicago calls HAR1F "a strong candidate gene
" for examining how changes in DNA sequence have contributed to the emergence of the distinctly human brain.
Some of the genes underlying this clinical variability have been uncovered after careful phenotypic (i.e., clinical) analysis, correlations with other distinct genetic disorders, and candidate gene
Progress in the area of plant genomics has been dramatic and the stage is set for efficient application of marker-assisted genetics, candidate gene
analysis, and molecular breeding.
Follistatin was a candidate gene
because of its role in regulating follicular growth and the secretion of follicle-stimulating hormone.
With similar arguments on MIS, for each candidate gene
g, the maximum function score (MFS) was calculated as follows:
SNP and candidate gene
annotation were retrieved using Ensembl annotation of Sscrofa 10.2 genome version in NCBI data base  while functional classification of candidate genes
with the target chromosomal segment was investigated using DAVID online annotation tools .
DENND1A gene in this locus was considered as a candidate gene
based on its role in regulating the production of endoplasmic reticulum amino-peptidase-1, used for membrane trafficking.18 Most recent GWAS and meta-analysis of candidate genes
showed significant association of two variants in the FBN3 gene with PCOS phenotype.19 FBN3 gene encodes fibrillin-3 protein.
Blood samples of the pro-band were collected and used for screening the hereditary cerebrovascular disease related genes, such as HTRA1, APP, CST3, and ITM2B, etc., and SNP genotyping assay were then conducted on family members after identifying the candidate gene
Melting curve, gel analysis, and sequences of each candidate gene
were analyzed to verify that a single PCR product was amplified for each set of primers.
Gene-Set Enrichment Analysis and Candidate Gene
The study will augment the information available and can be applied in future studies to determine the role of buffalo FASN gene as a candidate gene
marker for a milk-fat content.