The carcinoma was positive for cytokeratin (CK) 7 (Figure 1, G), CK18, mammaglobin (Figure 1, H), S100 protein (S100) (Figure 1, I), and signal transducer and activator of transcription 5a (STAT5a); focally positive for p63 protein (p63); and negative for gross cystic disease fluid protein 15 (GCDFP-15), discovered on GIST-1 (DOG1), smooth muscle actin (SMA), calponin
, and estrogen receptor/ progesterone receptor (ER/PR).
Neoplastic cells were negative for calponin
, ruling out myoepithelial cells.
They were verified by immunofluorescent staining of calponin
, a smooth muscle-specific protein.
Hematoxylin and eosin (H&E) stained slides were prepared and immunohistochemical (IHC) panel for vimentin (VIM), smooth muscle actin (SMA), desmin, CD10, CD34, CD68, leukocyte common antigen (LCA), chromogranin, S-100, cytokeratin (CK), B-cell lymphoma 2 (Bcl-2), thyroid transcription factor-1 (TTF-1), epithelial membrane antigen (EMA), calretinin, and calponin
These included smooth muscle myosin, calponin
, p63, CD10 and CK5/6 however, all of them failed to demonstrate MEC at the periphery of the lesion.
Differentiated or contractile VSMCs express unique markers, such as smooth muscle myosin heavy chain (SM-MHC), smooth muscle [alpha]-actin, transgelin, high molecular weight caldesmon (h-caldesmon) and calponin
, all of which are crucial for vasoregulation.
Five down-regulated miRNAs (bta-miR-424-5P, bta-miR-19b, bta-miR-29b, btamiR-30b, and bta-miR-2285) having sites binding to the mRNA of 12 distinct genes calponin
1 (CNN1), atypical chemokine receptor 3 (CXCR7), endothelin receptor type (BEDNRB), fibrinogen alpha chain (FGA), insulin like growth factor binding protein 3 (IGFBP3), regulator of G-protein signaling 2 (RGS2), periostin, osteoblast specific factor (POSTN), monoamine oxidase (AMAOA), collagen type IV alpha 6 (COL4A6), dehydrogenase/reductase (SDR family) member 3 (DHRS3), solute carrier family 22 (organic anion transporter), member 7 (SLC22A7).
Antibodies against calponin
, RUNX2 (runt-related transcription factor 2), and GRP78 (78-kDa glucose-regulated protein) were from Epitomic (Burlingame, USA).
The phenotype of the isolated cells was checked with phase contract microscopy and immunofluorescence (pancytokeratins [AE] 1/AE3 for UCs and smooth muscle actin [SMA] and calponin
for BSCs and SMCs).
0 A Structure of the Second Calponin
Homology Domain from the Actin-binding Region of the Dystrophin Homologue Utrophin.
The participation of calponin
in the cross talk between 20-hydroxyecdysone and juvenile hormone signaling pathways by phosphorylation variation.
Myoepithelial nature of the outer cell layer was confirmed by expression of smooth muscle actin and calponin