calcein


Also found in: Wikipedia.

calcein

A chelating agent which fluoresces brightly in the presence of bound Ca2+. The acetomethoxy derivative of calcein is used as a temporary label to test cell viability: once the calcein acetomethoxy complex enters healthy cells, intracellular esterases remove the acetomethoxy group, which fluoresces bright green—apparently in protest—something that dead cells, which lack esterases, cannot do.
Mentioned in ?
References in periodicals archive ?
were analyzed as previously described (14), and the levels of intracellular ROS and LIP were analyzed by measuring the MFI of 2'-7'dichlorofluorescein or calcein using a flow cytometer.
Protective effects of deferasirox and N-acetyl-L-cysteine on iron overload-injured bone marrow
5 [micro]M of calcein AM (Biotium, Fremont, CA) was added in each well and incubated for 30 minutes before the fluorescent intensities (ex/em: 495 nm/517 nm) were recorded by the plate reader.
Maintenance of Endothelial Cell Function in Liquid Based Antithrombotic Surface Coating
Limited Tenders are invited for Calcein Am Cell Permeate Dye And 28 More Items
Calcein Am Cell Permeate Dye And 28 More Items
Following incubation with the reagents cells must be washed to reduce the possibility of background fluorescence from unspecific extracellular binding of ethidium homodimer or hydrolysis in aqueous solution of AM calcein.
Obtencion y caracterizacion de andamios electrohilados a base de quitosano y fibroina del capullo (Bombyx mori)
Viability was defined as the number of H-33342 and calcein double-positive cells as determined by an automated algorithm described earlier (Stiegler et al.
Design of a high-throughput human neural crest cell migration assay to indicate potential developmental toxicants
2] results to the number of viable cells, calcein (10 [micro]g/ml) (Invitrogen Canada Inc.
Resveratrol, piperine and apigenin differ in their NADPH-oxidase inhibitory and reactive oxygen species-scavenging properties
Staining using calcein determined the amount of new calcification that occurred over 6 days at the end of the acclimation period.
Sea hare Aplysia punctata (Mollusca: Gastropoda) can maintain shell calcification under extreme ocean acidification
Calcein acetoxymethyl ester (CA-AM) was purchased from Setareh Biotech.
Modulation of iron metabolism by iron chelation regulates intracellular calcium and increases sensitivity to doxorubicin
Calcein as a fluorescent marker of otoliths of larval and juvenile fish.
Age, growth, and recruitment of larval and early juvenile Atlantic croaker (Micropogonias undulatus), determined from analysis of otolith microstructure
All rats were injected subcutaneously with calcein (10 mg/kg, Sigma Chemical Co.
Tanshinol Rescues the Impaired Bone Formation Elicited by Glucocorticoid Involved in KLF15 Pathway
Calcein Quenching to Measure Intracellular Iron Levels.
Hepcidin plays a key role in 6-OHDA induced iron overload and apoptotic cell death in a cell culture model of Parkinson's disease
Calcein staining of calcified structures in pearl oyster Pinctada margaritifera and the effect of food resource level on shell growth.
Growth performance comparison of Pinctada margaritifera juveniles produced by thermal shock or gonad scarification spawning procedures

Medical browser ?
Full browser ?