calcein


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calcein

A chelating agent which fluoresces brightly in the presence of bound Ca2+. The acetomethoxy derivative of calcein is used as a temporary label to test cell viability: once the calcein acetomethoxy complex enters healthy cells, intracellular esterases remove the acetomethoxy group, which fluoresces bright green—apparently in protest—something that dead cells, which lack esterases, cannot do.
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Sample incubation conditions were based on literature (9, 11, 12): 20 min at 37 [degrees]C for calcein AM and calcein violet, 60 min at 37 [degrees]C for CFSE, and 60 min at room temperature for di-8-ANEPPS and lactadherin.
Comparison of Generic Fluorescent Markers for Detection of Extracellular Vesicles by Flow Cytometry
Viability analysis was per formed on the same pictures using combined information from H-33342 and calcein channels.
A High-Throughput Approach to Identify Specific Neurotoxicants/Developmental Toxicants in Human Neuronal Cell Function Assays
Amplification can be detected through visualization with naked eye, due to the formation of a white precipitate of magnesium pyrophosphate or the change of color of the solution by using dyes (SYBR Green, calcein, HNB, picogreen).
Diagnostic methods for identification of root-knot nematodes species from Brazil/Metodos diagnosticos usados na identificacao de especies do nematoide das galhas do Brasil
Cytotoxicity studies using FACS (Figure 2S) showed no difference in viability measured by calcein staining, demonstrating that the membrane integrity of the cells was not compromised and that the MSCs remained highly viable after 24 hours of exposure to PAA-GNTs.
A New High-Performance Gadonanotube-Polymer Hybrid Material for Stem Cell Labeling and Tracking by MRI
Cell viability was verified in the agarose construct using calcein AM and propidium iodide fluorescent (live-dead) staining (Figure 3(a)).
Xenogenic Implantation of Equine Synovial Fluid-Derived Mesenchymal Stem Cells Leads to Articular Cartilage Regeneration
The cells were gently washed with serum-free medium, and calcein AM-labeled THP-1 cells (5 x [10.sup.4]/ml DMEM medium) (Sigma, USA) were then added to the endothelial cells.
Selenoprotein S Attenuates Tumor Necrosis Factor-[alpha]-Induced Dysfunction in Endothelial Cells
Tetracycline (12 mg/Kg body weight) on 7, 14, 21st days; Alizarin red S (30 mg/Kg body weight) on 28, 35 and 42nd days; Calcein (5 mg/Kg body weight) on 48, 56 and 63rd days.
Bone Remodeling Around Photochemical Fortified-calcium Silicate Implants in Long Term Rabbit Femur Model
With the purpose of material cytotoxicity assays performed with calcein and ethidium homodimer (Thermo[R]), different dilutions of silver nanoparticles synthesized by a green method were made using an extract of Annona muricata.
Cytotoxic and Bactericidal Effect of Silver Nanoparticles Obtained by Green Synthesis Method Using Annona muricata Aqueous Extract and Functionalized with 5-Fluorouracil
Calcein release behavior from liposomal bilayer; influence of physicochemical/mechanical/structural properties of lipids.
Encapsulation of the Piperine Present in Piper tuberculatum Species Using Multilamellar Vesicles and Determination of its Antioxidant Power/Encapsulacion de la piperine presente en la especie Piper tuberculatum utilizando vesiculas multilamelares y determinacion de su poder antioxidante
The hematopoietic progenitor cells (HPCs) ([Lineage.sup.-]/[Sca-1.sup.-]/[c-kit.sup.+], [LSK.sup.- ]) and HSCs ([Lineage.sup.-]/[Sca-1.sup.+]/[c-kit.sup.+]; [LSK.sup.+]) were analyzed as previously described (14), and the levels of intracellular ROS and LIP were analyzed by measuring the MFI of 2'-7'dichlorofluorescein or calcein using a flow cytometer.
Protective effects of deferasirox and N-acetyl-L-cysteine on iron overload-injured bone marrow
For live cell staining, 2.5 [micro]M of calcein AM (Biotium, Fremont, CA) was added in each well and incubated for 30 minutes before the fluorescent intensities (ex/em: 495 nm/517 nm) were recorded by the plate reader.
Maintenance of Endothelial Cell Function in Liquid Based Antithrombotic Surface Coating
Following incubation with the reagents cells must be washed to reduce the possibility of background fluorescence from unspecific extracellular binding of ethidium homodimer or hydrolysis in aqueous solution of AM calcein. The fluorescence of cells alone or cell suspension or tissue can be detected in the fluorescence microscope, fluorescence spectroscopy or microplate reader using appropriate filters.
Obtencion y caracterizacion de andamios electrohilados a base de quitosano y fibroina del capullo (Bombyx mori)

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