calcein


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calcein

A chelating agent which fluoresces brightly in the presence of bound Ca2+. The acetomethoxy derivative of calcein is used as a temporary label to test cell viability: once the calcein acetomethoxy complex enters healthy cells, intracellular esterases remove the acetomethoxy group, which fluoresces bright green—apparently in protest—something that dead cells, which lack esterases, cannot do.
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were analyzed as previously described (14), and the levels of intracellular ROS and LIP were analyzed by measuring the MFI of 2'-7'dichlorofluorescein or calcein using a flow cytometer.
5 [micro]M of calcein AM (Biotium, Fremont, CA) was added in each well and incubated for 30 minutes before the fluorescent intensities (ex/em: 495 nm/517 nm) were recorded by the plate reader.
Limited Tenders are invited for Calcein Am Cell Permeate Dye And 28 More Items
Following incubation with the reagents cells must be washed to reduce the possibility of background fluorescence from unspecific extracellular binding of ethidium homodimer or hydrolysis in aqueous solution of AM calcein.
Viability was defined as the number of H-33342 and calcein double-positive cells as determined by an automated algorithm described earlier (Stiegler et al.
2] results to the number of viable cells, calcein (10 [micro]g/ml) (Invitrogen Canada Inc.
Staining using calcein determined the amount of new calcification that occurred over 6 days at the end of the acclimation period.
All rats were injected subcutaneously with calcein (10 mg/kg, Sigma Chemical Co.
Calcein staining of calcified structures in pearl oyster Pinctada margaritifera and the effect of food resource level on shell growth.