If (and that's a critical caveat) the cDNA library
used for WES comes from the cell population of interest, this provides a snapshot not of the actual non-exonic sequences but of their significant effects.
The anagen skin cDNA library
of Liaoning cashmere goat was constructed according to the previous study .
Pre-procaeridin cDNAs were consistently cloned from the skin secretion cDNA library
, and each encoded a single copy of Caeridin, which were named Caeridin-1, S5-Caeridin-1, and Caeridin-a1 shown in Figure 1.
Briefly, yeast strain AH109 (Clontech) was sequentially transformed with the bait plasmid and a chicken cochlear cDNA library
in the HybriZAP two-hybrid vector .
Illumina cDNA library
preparation and sequencing: Approximately 30 g of total RNA with a concentration of [greater than or equal to] 250 ng/ L was used for cDNA synthesis and Solexa sequencing.
Ohotoko et al (2000) reported GH45 cellulase homologs from the symbiotic protists in the hindgut of termite Reticulitermes speratus using consensus PCR and cDNA library
Polymerase chain reactions for familial analysis and cDNA library
generation were carried out in a final volume of 20 [micro]l using the high-fidelity cloned Phusion polymerase (New England Biolabs), using appropriate buffers as the instructions indicated.
As a result, we obtained from ~3 to 15 million mapped reads for each cDNA library
The cDNA library
construction, 5'- and 3'-rapid amplification of cDNA ends (RACE), and homology cloning are main methods of gene cloning.
We sequenced unamplified poly(A)-tailed viral RNA using rapid cDNA library
preparation coupled with real-time data analysis to determine its potential application for pathogen genomic characterization.
Among these immunoreactive autoantigens, there are 2 proteins; in particular, Zuotin-related factor 1 (ZRF1) and R motif-containing protein 1 (KRR1) (clones KYMBC 29-88-1 and KY-MBC 18-53-1) were identified during the screening of cDNA library
from medullary breast carcinoma.
The cDNA library
was constructed after end repairing, A-Tailing, Adapter ligation and PCR.