cDNA cloning

cDNA cloning

a cloning technique in which mRNA is first converted to cDNA before insertion into a CLONING VECTOR. cDNA cloning is a particularly useful technique to employ when cloning eukaryotic genes containing INTRONS in BACTERIA. If the GENE is cloned directly in bacteria, the introns would inhibit its EXPRESSION. However, the mRNA, from which the cDNA derives, does not contain introns since these are removed during its formation. Thus cDNA will also lack introns and so its expression will not be prevented in bacteria.
References in periodicals archive ?
cDNA cloning and expression of a novel estrogen receptor P-subtype in goldfish (Carassius auratus).
cDNA cloning of NtCO1 gene from tobacco: Blast analysis was performed using S.
I decided first to develop from scratch a new cDNA cloning method that would allow for the high efficiency cloning of full-length cDNA of any size.
In this study, we report for the first time the purification, characterization, and cDNA cloning of a new anti-mycobacterial lectin protein isolated from red algae of Gelidium amansii.
Total RNA (2[micro]g) that was isolated from the hepatopancreas was reverse-transcribed using the SMART cDNA kit (Clonetech, USA) for cDNA cloning.
Isolation of full-length putative rat lhysophospholipase cDNA using improved methods for mRNA isolation and cDNA cloning.
For cDNA cloning, the RT-PCR amplified products of pooled whole animal and hemolymph samples of Population #2 and hemolymph from TSV-challenged survivors of cross #17 were run in a 1% agarose gel and eluted using Spin-X column (Costar, MA).
cDNA cloning, chromosomal mapping, and functional characterization of the human peroxisome proliferator activated receptor.
The two projects are random cDNA cloning, and in vitro expression and site-directed mutagenesis of cholinesterase; the latter project integrates the research and teaching of the principal investigator.
Unlike alternative approaches to gene discovery, such as cDNA cloning or whole genome sequencing, RAGE can be used to quickly identify these highly regulated genes.
Human natural resistance-associated macrophage protein: cDNA cloning, chromosomal mapping, genomic organization, and tissue-specific expression.