cDNA cloning

cDNA cloning

a cloning technique in which mRNA is first converted to cDNA before insertion into a CLONING VECTOR. cDNA cloning is a particularly useful technique to employ when cloning eukaryotic genes containing INTRONS in BACTERIA. If the GENE is cloned directly in bacteria, the introns would inhibit its EXPRESSION. However, the mRNA, from which the cDNA derives, does not contain introns since these are removed during its formation. Thus cDNA will also lack introns and so its expression will not be prevented in bacteria.
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References in periodicals archive ?
cDNA cloning and mRNA expression of neuropeptide Y in orange spotted grouper, Epinephelus coioides.
Molecular Cloning and Function Characterization in Feeding of Neuropeptide Y in Gibel Carp, Carassius auratus gibelio
Sagisaka et al., "cDNA cloning and gene expression of cecropin D, an antibacterial protein in the silkworm, Bombyx mori," Comparative Biochemistry and Physiology--B Biochemistry and Molecular Biology, vol.
Molecular Cloning, Bioinformatic Analysis, and Expression of Bombyx mori Lebocin 5 Gene Related to Beauveria bassiana Infection
cDNA cloning and expression analysis of CHS gene from wild potato.
Cloning and Sequence Analysis of Apomixis Related Gene MSI1 (Multicopy Suppressor of Ira1) in Taraxacum antungense
(1998) Purification, cDNA cloning, and expression of UDP-Gal:glucosylceramide [beta]-1,4-galactosyltransferase from rat brain.
Challenge to the suppression of tumor growth by the [beta]4-galactosyltransferase genes
cDNA cloning and expression of a novel estrogen receptor P-subtype in goldfish (Carassius auratus).
Molecular characterization and tissue distribution of estrogen receptor genes in domestic Yak
Tsunoo, "Purification, identification, and cDNA cloning of Jun a 2, the second major allergen of mountain cedar pollen," Biochemical and Biophysical Research Communications, vol.
Reduction of the number of major representative allergens: from clinical testing to 3-dimensional structures
For cDNA cloning of Pb[S.sub.26]-RNase gene, styles at bell stage (BS) were collected from "Hongpisu." For expression analysis of the Pb[S.sub.26]-RNase gene, styles of"Hongpisu" were collected at 96 h before anthesis (BA), 48 h BA, bell balloon stage (BBS), 12 h after pollination (AP), and 24 h AP, respectively.
Sequence characterization and spatiotemporal expression patterns of Pb[S.sub.26]-RNase gene in Chinese white pear (Pyrus bretschneideri)
cDNA cloning of NtCO1 gene from tobacco: Blast analysis was performed using S.
MOLECULAR CLONING AND ANALYSIS OF A CONSTANS HOMOLOG FROM NICOTIANA TABACUM
For example, in studies on cDNA cloning of autoantigens, this eukaryotic expression system, which often uses rabbit reticulocyte lysate, has been utilized in order to confirm whether patient's sera react to candidate clone's product and whether the clone product's mobility on SDS-PAGE is the same as the mobility of the endogenous cellular antigen [14-16].
A new ELISA for dermatomyositis autoantibodies: rapid introduction of autoantigen cDNA to recombinant assays for autoantibody measurement
cDNA Cloning. The primers used in the amplification of the goat PPAR[gamma] transcript sequence (PPARG) used for cDNA cloning are reported in Table 1.
PPAR[gamma] regulates genes involved in triacylglycerol synthesis and secretion in mammary gland epithelial cells of dairy goats
In this study, we report for the first time the purification, characterization, and cDNA cloning of a new anti-mycobacterial lectin protein isolated from red algae of Gelidium amansii.
Cloning and in vitro anti-mycobacterial activity of lectin protein in combination with streptomycin to increase sensitivity against Mycobacterium tuberculosis
Total RNA (2[micro]g) that was isolated from the hepatopancreas was reverse-transcribed using the SMART cDNA kit (Clonetech, USA) for cDNA cloning. Total RNA (2[micro]g) was reverse-transcribed using the PrimeScript RT-PCR kit (TaKaRa, Japan) for semi-quantitative RT-PCR (RT-PCR) analysis, or the PrimeScript Real-time PCR kit (TaKaRa, Japan) for real-time quantitative RT-PCR (qRT-PCR) analysis.
A novel TCTP gene from the crustacean Eriocheir sinensis: possible role involving metallic [Cu.sup.2+] stress

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