cDNA cloning

cDNA cloning

a cloning technique in which mRNA is first converted to cDNA before insertion into a CLONING VECTOR. cDNA cloning is a particularly useful technique to employ when cloning eukaryotic genes containing INTRONS in BACTERIA. If the GENE is cloned directly in bacteria, the introns would inhibit its EXPRESSION. However, the mRNA, from which the cDNA derives, does not contain introns since these are removed during its formation. Thus cDNA will also lack introns and so its expression will not be prevented in bacteria.
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References in periodicals archive ?
cDNA cloning and expression analysis of CHS gene from wild potato.
Cloning and Sequence Analysis of Apomixis Related Gene MSI1 (Multicopy Suppressor of Ira1) in Taraxacum antungense
cDNA cloning and expression of a novel estrogen receptor P-subtype in goldfish (Carassius auratus).
Molecular characterization and tissue distribution of estrogen receptor genes in domestic Yak
cDNA cloning of NtCO1 gene from tobacco: Blast analysis was performed using S.
MOLECULAR CLONING AND ANALYSIS OF A CONSTANS HOMOLOG FROM NICOTIANA TABACUM
I decided first to develop from scratch a new cDNA cloning method that would allow for the high efficiency cloning of full-length cDNA of any size.
Functional cDNA expression cloning: pushing it to the limit
In this study, we report for the first time the purification, characterization, and cDNA cloning of a new anti-mycobacterial lectin protein isolated from red algae of Gelidium amansii.
Cloning and in vitro anti-mycobacterial activity of lectin protein in combination with streptomycin to increase sensitivity against Mycobacterium tuberculosis
Total RNA (2[micro]g) that was isolated from the hepatopancreas was reverse-transcribed using the SMART cDNA kit (Clonetech, USA) for cDNA cloning.
A novel TCTP gene from the crustacean Eriocheir sinensis: possible role involving metallic [Cu.sup.2+] stress
Isolation of full-length putative rat lhysophospholipase cDNA using improved methods for mRNA isolation and cDNA cloning.
Extraction of functional total RNA from Cayratia trifolia
For cDNA cloning, the RT-PCR amplified products of pooled whole animal and hemolymph samples of Population #2 and hemolymph from TSV-challenged survivors of cross #17 were run in a 1% agarose gel and eluted using Spin-X column (Costar, MA).
Linkage mapping of developmentally expressed cDNAS containing AT-rich elements in shrimp (Litopenaeus vannamei)--differential expression after challenge with Taura Syndrome virus (TSV)
cDNA cloning, chromosomal mapping, and functional characterization of the human peroxisome proliferator activated receptor.
Is Peroxisome Proliferation an Obligatory Precursor Step in the Carcinogenicity of Di(2-ethylhexyl)phthalate (DEHP)?
The two projects are random cDNA cloning, and in vitro expression and site-directed mutagenesis of cholinesterase; the latter project integrates the research and teaching of the principal investigator.
A COLLABORATIVE RECOMBINANT DNA TECHNOLOGY COURSE WITH LABORATORY
Unlike alternative approaches to gene discovery, such as cDNA cloning or whole genome sequencing, RAGE can be used to quickly identify these highly regulated genes.
Athersys announces filing of United States patent applications covering over 10,000 novel gene sequences
Human natural resistance-associated macrophage protein: cDNA cloning, chromosomal mapping, genomic organization, and tissue-specific expression.
Comparative Genomics and Host Resistance against Infectious Diseases

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