cDNA cloning

cDNA cloning

a cloning technique in which mRNA is first converted to cDNA before insertion into a CLONING VECTOR. cDNA cloning is a particularly useful technique to employ when cloning eukaryotic genes containing INTRONS in BACTERIA. If the GENE is cloned directly in bacteria, the introns would inhibit its EXPRESSION. However, the mRNA, from which the cDNA derives, does not contain introns since these are removed during its formation. Thus cDNA will also lack introns and so its expression will not be prevented in bacteria.
References in periodicals archive ?
cDNA cloning and mRNA expression of neuropeptide Y in orange spotted grouper, Epinephelus coioides.
Sagisaka et al., "cDNA cloning and gene expression of cecropin D, an antibacterial protein in the silkworm, Bombyx mori," Comparative Biochemistry and Physiology--B Biochemistry and Molecular Biology, vol.
(1998) Purification, cDNA cloning, and expression of UDP-Gal:glucosylceramide [beta]-1,4-galactosyltransferase from rat brain.
cDNA cloning and expression of a novel estrogen receptor P-subtype in goldfish (Carassius auratus).
Tsunoo, "Purification, identification, and cDNA cloning of Jun a 2, the second major allergen of mountain cedar pollen," Biochemical and Biophysical Research Communications, vol.
For cDNA cloning of Pb[S.sub.26]-RNase gene, styles at bell stage (BS) were collected from "Hongpisu." For expression analysis of the Pb[S.sub.26]-RNase gene, styles of"Hongpisu" were collected at 96 h before anthesis (BA), 48 h BA, bell balloon stage (BBS), 12 h after pollination (AP), and 24 h AP, respectively.
cDNA cloning of NtCO1 gene from tobacco: Blast analysis was performed using S.
For example, in studies on cDNA cloning of autoantigens, this eukaryotic expression system, which often uses rabbit reticulocyte lysate, has been utilized in order to confirm whether patient's sera react to candidate clone's product and whether the clone product's mobility on SDS-PAGE is the same as the mobility of the endogenous cellular antigen [14-16].
cDNA Cloning. The primers used in the amplification of the goat PPAR[gamma] transcript sequence (PPARG) used for cDNA cloning are reported in Table 1.
In this study, we report for the first time the purification, characterization, and cDNA cloning of a new anti-mycobacterial lectin protein isolated from red algae of Gelidium amansii.
Total RNA (2[micro]g) that was isolated from the hepatopancreas was reverse-transcribed using the SMART cDNA kit (Clonetech, USA) for cDNA cloning. Total RNA (2[micro]g) was reverse-transcribed using the PrimeScript RT-PCR kit (TaKaRa, Japan) for semi-quantitative RT-PCR (RT-PCR) analysis, or the PrimeScript Real-time PCR kit (TaKaRa, Japan) for real-time quantitative RT-PCR (qRT-PCR) analysis.