and mRNA expression of neuropeptide Y in orange spotted grouper, Epinephelus coioides.
Sagisaka et al., "cDNA cloning
and gene expression of cecropin D, an antibacterial protein in the silkworm, Bombyx mori," Comparative Biochemistry and Physiology--B Biochemistry and Molecular Biology, vol.
and expression analysis of CHS gene from wild potato.
(1998) Purification, cDNA cloning
, and expression of UDP-Gal:glucosylceramide [beta]-1,4-galactosyltransferase from rat brain.
and expression of a novel estrogen receptor P-subtype in goldfish (Carassius auratus).
Tsunoo, "Purification, identification, and cDNA cloning
of Jun a 2, the second major allergen of mountain cedar pollen," Biochemical and Biophysical Research Communications, vol.
For cDNA cloning
of Pb[S.sub.26]-RNase gene, styles at bell stage (BS) were collected from "Hongpisu." For expression analysis of the Pb[S.sub.26]-RNase gene, styles of"Hongpisu" were collected at 96 h before anthesis (BA), 48 h BA, bell balloon stage (BBS), 12 h after pollination (AP), and 24 h AP, respectively.
of NtCO1 gene from tobacco: Blast analysis was performed using S.
For example, in studies on cDNA cloning
of autoantigens, this eukaryotic expression system, which often uses rabbit reticulocyte lysate, has been utilized in order to confirm whether patient's sera react to candidate clone's product and whether the clone product's mobility on SDS-PAGE is the same as the mobility of the endogenous cellular antigen [14-16].
. The primers used in the amplification of the goat PPAR[gamma] transcript sequence (PPARG) used for cDNA cloning
are reported in Table 1.
In this study, we report for the first time the purification, characterization, and cDNA cloning
of a new anti-mycobacterial lectin protein isolated from red algae of Gelidium amansii.
Total RNA (2[micro]g) that was isolated from the hepatopancreas was reverse-transcribed using the SMART cDNA kit (Clonetech, USA) for cDNA cloning
. Total RNA (2[micro]g) was reverse-transcribed using the PrimeScript RT-PCR kit (TaKaRa, Japan) for semi-quantitative RT-PCR (RT-PCR) analysis, or the PrimeScript Real-time PCR kit (TaKaRa, Japan) for real-time quantitative RT-PCR (qRT-PCR) analysis.