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We also isolated a cDNA clone encoding a hemichordate (Ptychodera flava) homolog (PfBra) of the Brachyury gene and examined its expression pattern during embryogenesis (17).
The nucleotide sequence of the cDNA clone for CiEnds1 will appear under the accession number of AB010895 in the DDBJ, EMBL, and GenBank nucleotide sequence databases.
In Figure 3 the aligned nucleotide sequence data obtained from 5[prime] RACE, RT-PCR with degenerate oligonucleotide primers, and from the largest cDNA clone isolated are presented.
A total of 66 genomic and 34 cDNA clone sequences were used to detect RFLPs between the 28 cultivars (Table 2).
The quality of each cDNA library was assayed by probing nitrocellulose plaque lifts for representation of actin-complementary sequences using the SpCA8 cytoactin cDNA clone. Phage transfer was performed for 1 min at room temperature, and filters were sequentially placed for 3 min each onto sheets of 3MM paper saturated with the following solutions: (1) 0.5 N sodium hydroxide and 1.5 M sodium chloride, (2) 10% SDS, (3) 0.5 M Tris-HCl pH = 8.0 and 1.5 M NaCl, and (4) 2 X SSC.
A cDNA clone for cyclophilin from Griffithsia japonica and phylogenetic analysis of cyclophilins.
While human and mouse hdac6 have been well characterized and annotated due to extensive literature and experimental evidences available, there is no information about rat hdac6, due to unavailability of cDNA clone [6].
Construction of Papaya ringspot virus (PRSV) intermediate cDNA clones: The intermediate clone, pBS-HP-EX was constructed by ligating a cDNA fragment which contained the HC-Pro gene flanked by partial P1 and P3 genes from the full-length cDNA clone of Papaya ringspot virus type-W Thailand isolate, pSA1164 (Yap et al., 2009) into the pBlueScriptKS (Stratagene) vector.
coli / Bacillus), Yeast Expression Systems (P pastoris / S cerevisiae), Baculovirus-insect Cell Expression Systems, Mammalian Expression Systems (CHO / 293)), Membrane Protein (Custom Membrane Protein Production, Cell Line Development and Membrane Preparation, Custom Lipoparticle Containing Membrane Protein, Lipid-based Protein Immobilization), PEGylation Services, Directed Evolution, Protein Engineering, Mutagenesis Services (Site-Directed Mutagenesis, Site-Saturation Mutagenesis, Controlled Randomization Service), Protein Microarray , Custom cDNA clone etc.
This is not a problem if oligonucleotide hybridization (21) or antibody detection (22-24) is intended to be used to screen for a particular cDNA clone, but it is not a trivial issue if the use of selection or the screening of cDNA clones based on the functions of the proteins they encode is envisioned to isolate a particular cDNA clone.
Amino acid sequences of the isolated peptides derived from algae lectin corresponded exactly to the protein sequence deduced from the cDNA sequence, clearly indicating that the isolated cDNA clone codes for lectin protein.