Predicted amino acid sequence of recombinant SPLUNC1 cDNA
In conclusion, we characterized the cDNA
encoding SS1 in C.
Colony PCR was used to confirm the size of inserted fragments in the library by the primers (cDNA
F: CCATTGTGTTGGTACCCGG; cDNA
After overnight incubating at 37[degrees]C, colony-PCR using gene-specific primers and conditions similar to PCR reaction for amplification of CD 19 cDNA
were applied on white colonies.
After cloning sequencing and alignment with cDNA
the length of genomic sequence was 1460 bp.
The products of double-stranded cDNA
synthesis (1500 ng) were analyzed with 2D-SDE on 6% polyacrylamide gels with 7 mol/L urea (14).
This allows oligo dT to anneal to mRNA and to serve as the primer from which the first strand of cDNA
Although currently retroviral reverse transcriptases are routinely used to synthesize cDNA
copies of various mRNAs, it was S.
were synthesized with 1 [mu]g of total RNA using SMART Polymerase chain reaction (PCR) cDNA
synthesis kit (Clontech Laboratories, Inc.
A good quality total RNA was used for the first strand cDNA
synthesis using SuperScript III First-Strand Synthesis System for RT-PCR (Invitrogen, USA) according to the manufacturer recommendations.
The use of viruses as vectors for cDNA
libraries overcomes the difficulty of isolating antigens in tumor cells by giving the immune system a more co-plete picture of the cancerous invader.
7 times higher than that achieved with the wild type cDNA
, while a fully synthetic (FS) cDNA
displayed a 3-fold higher yield and a 2.