(2009) for freezing of buffalo sperm) of
butylated hydroxytoluene (W218405, Sigma-Aldrich Co, Australia), which was dissolved in 0.25% (v/v) dimethyl sulfoxide (DMSO) before addition to extender.
Out of all the screened compounds, 18 (IC50 = 55.2 +- 1.2 uM) was found to be the most potent if compared with standard
butylated hydroxytoluene (BHT) (IC50 = 128.8 +- 2.1 uM).
1, 1-diphenyl-2-picrylhydrazyl (DPPH), Dimethyl sulfoxide (DMSO), BHT (
Butylated Hydroxytoluene) (standard)- 1.6 mg/mL in methanol and samples desired concentration from 1 mg/mL-max of 5 mg/mL (in/DMSO) were used in this study.
The DPPH radical-scavenging activities of different concentrations of Sample 1, Sample 2, and the three standard solutions (butylated hydroxyanisole (BHA),
butylated hydroxytoluene (BHT), and L-ascorbic acid (L-AA)) are shown in Figure 1.
Hydrazine hydrate, phosphate buffer solution, pyrrole, graphene oxide, and
butylated hydroxytoluene were obtained from Sigma-Aldrich (MO, USA).
Antioxidant test results of the sample and standards HPSA (1) FRAP (2) DPPH (1) Propolis 11.72[+ or -]0.04 90.73[+ or -]0.24 18.34[+ or -]0.08 BHA 184.13[+ or -]1.19 92.02[+ or -]0.80 8.53[+ or -]0.39 BHT 147.49[+ or -]0.09 54.16[+ or -]0.10 9.01[+ or -]0.02 TOC 216.26[+ or -]0.47 32.98[+ or -]0.21 11.97[+ or -]0.07 TPC (3) TFC (4) MCA (2) Propolis 10673.4[+ or -]3.30 170.65[+ or -]1.12 89.69[+ or -]0.12 BHA - - 89.95[+ or -]0.05 BHT - - 86.26[+ or -]0.15 TOC - - 93.41[+ or -]0.06 (1) [SC.sub.50] ([micro]g/mL) (2) % activity (3) mg GAE/100 g of PS (4) mg QE/100 g of PS BHA: butylated hydroxyanisole; BHT:
butylated hydroxytoluene; TOC: [alpha]-tocopherol
Survival and fertility of boar spermatozoa after freeze-thawing in extender supplemented with
butylated hydroxytoluene. J Androl 25: 397-405.
BHA (butylated hydroxyanisole) and BHT (
butylated hydroxytoluene)
Addition of synthetic antioxidants, such as butylated hydroxyanisole (BHA),
butylated hydroxytoluene (BHT), and tert-butylhydroquinone (TBHQ), is one of the most effective and popular methods to prevent oxidation and the development of off-flavors (Halliwell and Gutteridge, 1985).
Utilization of synthetic antioxidants, that is, citric acid, propyl gallate, butylated hydroxyanisole (BHA), and
butylated hydroxytoluene (BHT) in foods, leads to many side effects.
Sodium hydroxide and anhydrous sodium sulfate were obtained from Pronalab[R] (Lisbon, Portugal); boron trifluoride in methanol (14% in methanol) and
butylated hydroxytoluene (BHT) ([greater than or equal to] 99%) were from Sigma-Aldrich[R] (St.
The mixture was incubated at 37 [degrees]C for 60 min, after which, 50 [micro]L of 2.0%
butylated hydroxytoluene (BHT) was added to each tube, followed by 1 mL of 2.8% trichloroacetic acid (TCA) and 1 mL of 1.0% 2-thiobarbituric acid (TBA) in 0.05 M NaOH.
