For example, the Ngari virus genome consists of segments from BATV and Bunyamwera virus. Ngari virus is associated with outbreaks of hemorrhagic fever in humans and shows a clinical spectrum different from that of both parent viruses (2).
For example, a virus from the same serogroup, Bunyamwera virus, was recently associated with neurologic disease and abortion in horses (15).
To their surprise, they found that mosquitoes collected near Potosi, Mo., harbored a previously unidentified member of the Bunyamwera virus
The 3 segmented RNAs of Bunyamwera virus
were used as outgroups to root the trees.
The largest genus in the family Bunyaviridae, the genus Orthobunyavirus was originally named Bunyavirus, for the type species Bunyamwera virus
, first isolated in 1943 from the eponymous town in western Uganda.
Ngari virus is a Bunyamwera virus
reassortant that can be associated with large outbreaks of hemorrhagic fever in Africa.
Sequence analysis showed that NRIV is a reassortant between Bunyamwera virus
(BUNV) and Batai virus (BATV), both from the genus Orthobunyavirus.
Serum specimens were tested for immunoglobulin M (IgM) and IgG antibodies specific for DENV, West Nile virus (WNV), Wesselsbron virus, Rift Valley fever virus, Bunyamwera virus, and CHIKV by IgM-antibody capture (MAC-ELISA) and IgG sandwich ELISA, respectively (11).
([dagger]) DENV, West Nile virus, Wesselsbron virus, Rift Valley fever virus, Bunyamwera virus, and CHIKV antibodies tested.
The first sample (November 3, 2004) contained no antibodies (OD ratio <2) to dengue viruses, West Nile virus, Wesselsbron virus, Rift Valley fever virus, Bunyamwera virus
, or Chikungunya virus (CHIKV).
Indirect immunofluorescence (IF) was performed on C6/36 cells with DENV-1, -2, -3, and -4 serotype--specific monoclonal antibodies (kindly provided by Nick Karabatsos, Centers for Disease Control and Prevention, Fort Collins, Colorado); on mouse hyperimmune ascitic fluids for West Nile virus and Bunyamwera virus; and on grouping fluids for alphaviruses, phleboviruses, and California serogroup viruses.
Moreover, IF tests were negative when antibody to West Nile virus, Toscana virus, Bunyamwera virus, alphaviruses, phleboviruses and California serogroup viruses was used.