bulked segregant analysis

bulked segregant analysis

A strategy used for rapidly mapping plant genomes, which is suitable for monogenic qualitative traits. When DNA of ten plants are bulked into one pool, all alleles must be present; two bulked pools of segregants differing for one trait will differ only at the locus harbouring that trait.
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Bulked segregant analysis (BSA) is a method that can be used for the rapid screening and verification of useful molecular markers during the selection and breeding process.
Bulked segregant analysis identifies molecular markers associated with early bulking in cassava (Manihot esculenta Crantz).
Keywords: Sweet sorghum; Bulked segregant analysis; Random amplified polymorphic DNA; Sequence characterized amplified regions (SCAR); Marker-assisted selection.
Bulked segregant analysis [17] is a method used for rapidly identifying markers linked to any specific gene or genomic region.
and Polito,V.S., (1994) "Identification of a RAPD marker linked to sex determination in Pistacia vera using bulked segregant analysis".
A bulked segregant analysis (BSA) (Michelmore et al., 1991) was completed to identify AFLP markers near the LG I protein QTL to further saturate the region with molecular markers and increase mapping resolution.
El analisis de segregantes agrupados (Bulked Segregant Analysis o BSA) fue desarrollado por Michelmore et al.
The DNA marker for the velvet hairiness trait was identified by RAPD technique using bulked segregant analysis. A cross of velvet hairy (densely hairy) and sparsely hairy genotypes was made and segregating F2 generation was developed for DNA marker studies.
The identification of markers associated with different disease resistance genes has been achieved by the use of bulked segregant analysis (BSA) in combination with molecular markers in various crop plants.
Simple sequence repeat (SSR) markers were used in three different approaches to search for phytate loci in the Boggs-RR x CX1834-1-2 population: (i) bulked segregant analysis (BSA; Michelmore et al., 1991), (ii) a modified BSA method which is described later, and (iii) a whole-genome scan of a subset of 94 random individuals.
Bulked segregant analysis (Michelmore et al., 1991), simple F tests, and interval mapping (Lander and Botstein, 1989) are useful techniques for identifying molecular markers linked to a specific gene or region of the genome.
Abbreviations: AFLP, amplified fragment length polymorphism; BSA, bulked segregant analysis; cM, centimorgans; FI, female index; LG, linkage group; LOD, likelihood of odds; PI, plant introduction; QTL, quantitative trait loci; SCN, soybean cyst nematode; SSR, simple sequence repeat.