bulked segregant analysis

bulked segregant analysis

A strategy used for rapidly mapping plant genomes, which is suitable for monogenic qualitative traits. When DNA of ten plants are bulked into one pool, all alleles must be present; two bulked pools of segregants differing for one trait will differ only at the locus harbouring that trait.
References in periodicals archive ?
Bulked segregant analysis (BSA) is a method that can be used for the rapid screening and verification of useful molecular markers during the selection and breeding process.
BSA and molecular markers screening for salt stress tolerant mutant of Petunia obtained in in vitro culture/ Rastreamento de BSA e marcadores moleculares para mutante tolerante ao estresse salino de Petunia obtido em cultura in vitro
Bulked segregant analysis identifies molecular markers associated with early bulking in cassava (Manihot esculenta Crantz).
GENOTYPE X ENVIRONMENT INTERACTION AND STABILITY ANALYSIS OF CASSAVA GENOTYPES AT DIFFERENT HARVEST TIMES
Keywords: Sweet sorghum; Bulked segregant analysis; Random amplified polymorphic DNA; Sequence characterized amplified regions (SCAR); Marker-assisted selection.
Development of A High Sugar Trait-Related SCAR Marker in Sweet Sorghum
Bulked segregant analysis [17] is a method used for rapidly identifying markers linked to any specific gene or genomic region.
Identification and validation of a new male sex-specific ISSR marker in pointed gourd (Trichosanthes dioica Roxb.)
and Polito,V.S., (1994) "Identification of a RAPD marker linked to sex determination in Pistacia vera using bulked segregant analysis".
Development of biochemical and molecular markers for polygamodioecious character in Simarouba glauca DC
A bulked segregant analysis (BSA) (Michelmore et al., 1991) was completed to identify AFLP markers near the LG I protein QTL to further saturate the region with molecular markers and increase mapping resolution.
Fine mapping of a seed protein QTL on soybean linkage group I and its correlated effects on agronomic traits
El analisis de segregantes agrupados (Bulked Segregant Analysis o BSA) fue desarrollado por Michelmore et al.
Analisis de segregantes agrupados (BSA) para la deteccion de AFLPS ligados al gen de resistencia a PVX en Solanum commersonii (1)
The DNA marker for the velvet hairiness trait was identified by RAPD technique using bulked segregant analysis. A cross of velvet hairy (densely hairy) and sparsely hairy genotypes was made and segregating F2 generation was developed for DNA marker studies.
TAGGING GENES FOR VELVET HAIRINESS IN UPLAND COTTON
The identification of markers associated with different disease resistance genes has been achieved by the use of bulked segregant analysis (BSA) in combination with molecular markers in various crop plants.
Molecular Characterization of Pea for Resistance to Pseudomonas syringae pv pisi
Simple sequence repeat (SSR) markers were used in three different approaches to search for phytate loci in the Boggs-RR x CX1834-1-2 population: (i) bulked segregant analysis (BSA; Michelmore et al., 1991), (ii) a modified BSA method which is described later, and (iii) a whole-genome scan of a subset of 94 random individuals.
Genetic mapping of loci associated with seed phytic acid content in CX1834-1-2 soybean
Bulked segregant analysis (Michelmore et al., 1991), simple F tests, and interval mapping (Lander and Botstein, 1989) are useful techniques for identifying molecular markers linked to a specific gene or region of the genome.
RAPD and SCAR markers linked to an introgressed gene conditioning resistance to Peronospora tabacina D.B. Adam. in tobacco
Abbreviations: AFLP, amplified fragment length polymorphism; BSA, bulked segregant analysis; cM, centimorgans; FI, female index; LG, linkage group; LOD, likelihood of odds; PI, plant introduction; QTL, quantitative trait loci; SCN, soybean cyst nematode; SSR, simple sequence repeat.
Localization of two loci that confer resistance to soybean cyst nematode from Glycine soja PI 468916
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