buffy coat


Also found in: Dictionary, Thesaurus, Encyclopedia, Wikipedia.

coat

 [kōt]
1. a membrane or other structure covering or lining a part or organ; in anatomic nomenclature called tunica.
2. the layer or layers of protective protein surrounding the nucleic acid in a virus. See also capsid.
buffy coat the thin yellowish layer of leukocytes overlying the packed erythrocytes in centrifuged blood.

buff·y coat

the upper, lighter portion of the blood clot (coagulated plasma and white blood cells), occurring when coagulation is delayed so that the red blood cells have had time to settle; the portion of centrifuged, anticoagulated blood that contains leukocytes and platelets.

buffy coat

The flavescent (yellow-white) band of cells and debris present between the upper layer of plasma and the lower layer of RBCs when whole blood is spun at 5000 RPM, which corresponds to WBCs.

buffy coat

Lab medicine The flavescent yellow-white band of cells and debris present between the upper layer of plasma and the lower layer of RBCs, when whole blood is spun at 5000 RPM, which corresponds to WBCs

buf·fy coat

(bŭf'ē kōt)
The light-colored layer of blood that is seen when anticoagulated blood is centrifuged or allowed to stand. It appears as a layer between the plasma and eythrocytes and is composed of leukocytes and platelets.

buffy coat

Enlarge picture
BUFFY COAT
A light stratum of blood seen when the blood is centrifuged or allowed to stand in a test tube. The red blood cells settle to the bottom and, between the plasma and the red blood cells, a light-colored layer contains mostly white blood cells. Platelets are at the top of this coat; the next layers, in order, are lymphocytes and monocytes; granulocytes; and reticulocytes. In normal blood, the buffy coat is barely visible; in leukemia and leukemoid reactions, it is much larger. See: illustration

buffy coat

The creamy layer of white blood cells that forms between the PLASMA and the column of packed red cells when anticoagulated blood is centrifuged. Practical use is made of this layering in the production of buffy coat-poor red cell transfusion fluids in cases in which it is considered that the risk of post-operative infection from the immunosuppressive effect of white cells in transfused blood is excessive.

Buffy coat

The thin layer of concentrated white blood cells that forms when a tube of blood is spun in a centrifuge.
Mentioned in: Leukemia Stains
References in periodicals archive ?
The buffy coat layer was carefully removed and transferred to a 1.5-mL tube for immediate processing to genomic DNA (gDNA).
The centrifugal quantitative buffy coat combines an acridine orange-coated capillary tube and an internal float to separate layers of WBC and platelets using centrifugation.
detection, +([section]) y+([paragraph]) Giemsa-stained blood smear Quantitative buffy coat ND ND ELISA anti-B.
Only 2 buffy coat samples were available given the low number of confirmed samples available worldwide.
Buffy coat analysis, a pictorial review (privately published by Becton Dickinson), 1983.
Acknowledgments: We would like to thank James Ford in the flow laboratory for preparing the buffy coats used in this study.
DNA extracted from the buffy coat and the tumor tissues of the HCC patients was genotyped with the Affymetrix Genome-Wide Human SNP Array 6.0 system, as previously described (23 ).
Buffy coat samples, washed twice with PBS by centrifugation at 120g for 10 min to remove the culture media, were resuspended in PBS saturated with [Ca.sup.2+].
Contract award notice: bc (buffy coat) trombokontsentraatide valmistamise ssteemide ostmine koos automatiseeritud trombokontsentraatide valmistamise seadme kasutamisega.
After centrifugation, buffy coat of the serum sample was removed and stored at -80[degrees]C (1).
For a given protocol (microfluidics or buffy coat), the correlation coefficients of the gene expression profiles between the individuals were calculated in a pairwise fashion and then averaged.
After centrifugation, most of the plasma is removed, leaving an amount approximately equal to the amount of buffy coat. The remaining plasma and buffy coat are then separated from the red cell layer and mixed.