For the analysis of the mtDNA D-loop region, fifteen unrelated, Nili-Ravi type buffaloes
were selected from the irrigated agriculture belt of the Nili and Ravi Bar regions of the Punjab province in Pakistan.
They called the animal a "buffalo" because it reminded them of buffaloes
in other parts of the world.
This is hub of breeding buffaloes
and a big market for supplying milk to all the areas of twin cities of Rawalpindi and Islamabad.
Today the Crewses own 16 buffaloes
, including the massive, 2,400-pound Beast, who joined their family when Needmore Buffalo Farm closed.
This was land we couldn't do anything with until we brought in buffalo," says Wilfried Merle, owner of Hato Rio de Agua, a marshy 1,000-hectare ranch where some 700 buffaloes
splash about in swampy glory "We tried cattle, rice and even capybaras.